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Objective research ambroxol hydrochloride cable joint inhaled cloth budesonide sensitized rats airway inflammation and lung tissue levels of oxidative stress, ambroxol hydrochloride further explore the role of adjuvant therapy for asthma. Methods 32 male Wistar rats were randomly divided into a control group, the intervention group (BUD) of the sensitized group, budesonide and ambroxol Sorbian budesonide intervention group (AMB BUD). Ovalbumin (OVA) sensitization and inhalation challenge Preparation of rat asthma model. Determination of the rats in bronchoalveolar lavage fluid (ABLF) cell count and classification; portion of lung HE staining the pathology observed; colorimetric determination of reactive oxygen (ROS) in lung tissue, reduced glutathione (GSH in situ hybridization) content; immunohistochemical determination of lung tissue γ-glutamylcysteine ??synthetase (γ-GCS) and nuclear factor-related factor 2 (Nrf2) expression; lung tissue gamma-GCS-hmRNA and expression of Nrf2mRNA. 1, lung tissue inflammation-related indicators: the total number of cells in the the ① sensitized group BALF eosinophils (EOS) and lymphocytes (L), the proportion was significantly higher than those of other groups; AMB BUD in BALF total cells, eosinophils granulocytes (EOS) and lymphocytes (L) proportion than in the BUD group decreased significantly among groups differences were statistically significant (all P lt; 0.05). ② lung HE staining results: In addition to the control group in each experimental group have different levels of lung tissue damage, showed bronchial epithelial cell swelling, loss, airway smooth muscle layer and reticular basement membrane thickening, bronchial contraction in wall thickening, luminal narrowing, wall and peritubular infiltration of a large number of inflammatory cells, mainly eosinophils and lymphocytes. Its severity descending order sensitized group, BUD group the AMB BUD group and control group. 2, the oxidative stress indicators: ① sensitized lung tissue levels of ROS compared with the other three groups significantly increased, GSH decreased significantly compared to the other three groups; significantly lower compared with the BUD group AMB the BUD group of lung tissue ROS, GSH group compared to BUD obvious liter high (all P lt; 0.01). ② sensitized group gamma-GCS-hmRNA gamma-GCS protein expression was significantly higher than in the other groups (all P lt; 0.01); AMB the BUD group of gamma-GCS-hmRNA gamma-GCS protein expression were higher than BUD group (all P lt; 0.01); above both expression BUD group and the control group, the difference was not obvious, was not statistically significant (both P gt; 0.05). No significant difference (P gt; 0.05); Nrf2 expression of sensitized group was significantly higher than other groups (all P lt; 0.01); ③ Nrf2mRNA expression among the experimental group compared to BUD group AMB BUD group Nrf2 expression was significantly higher (P lt; 0.01); BUD group and the control group, the expression of Nrf2 was no significant difference (P gt; 0.05). 3, linear correlation analysis showed that: the total number of cells in BALF eosinophils (EOS) and lymphocytes (L), the proportion of the levels of ROS are highly correlated (the r order 0.999,0.999,0.990), and GSH content was highly negative correlation (r order -0.877, -0.877, -0.838); y-GCS, γ-GCS-hmRNA γ-GCS protein expression levels of ROS content was positively correlated (the r order 0.825,0.799,0.873), and GSH The content was negatively correlated (r -0.652, -0.620, -0.702). Conclusion 1. Lungs of sensitized rats oxidant / antioxidant imbalance, has an important role in the pathogenesis of asthma. Ambroxol hydrochloride joint inhaled corticosteroids sensitized rats airway inflammation and lung tissue can significantly reduce the level of oxidative stress, has a synergistic therapeutic effect in acute asthma attacks. One of the mechanisms of ambroxol hydrochloride joint inhaled corticosteroids can increase the expression of Nrf2 and γ-GCS, may play its anti-inflammatory antioxidant effects.
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