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Studies on Assay Method of Flavonoids and Ultrasound-Assisted Extraction Process in Oxytropis Plants

Author: LiuZuo
Tutor: ZhaoBaoYu
School: Northwest University of Science and Technology
Course: Clinical Veterinary Medicine
Keywords: Oxytropis Flavone Determination Extraction
CLC: TQ461
Type: Master's thesis
Year: 2010
Downloads: 342
Quote: 4
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Abstract


There are a resource-rich of Oxytropis on the earth , many of them are used for anti-inflammatory and detoxification and antibacterial and sedative and analgesic, the physiological activity of them contains flavonoids and triterpene-saponins and alkaloids et al. Flavonoids have effects of anti-ischemic against myocardial ischemia and antiarrhythmic and analgesia and protection for the liver and anti-virus and droping blood lipid and inhibiting platelet aggregation and anti-tumor activity et al. its have been given a high priority and concern by world governments and the pharmaceutical and food industries. At present the studies only focus on its toxic components, while there were a less report on Oxytropis extraction of total flavonoids, which greatly affected Oxytropis effective use. Therefore, This experiment was to conduct extraction and determination of flavonoids of Oxytropis, the conclusions is following:1. Studies of pre-testing of system on Oxytropis the chemical constituents and of pre-testing of specific on flavonoids in Oxytropis plants. The results show that O. deflixa and O. glocialis contain mostly sugar and polysaccharides and glycosides and amino acids and peptides and proteins, and organic acids and phenols and tannins and flavonoids and their glycosides, and alkaloids and steroids and terpenoids and coumarin and grease, etc., however, Anthraquinones and cardiac glycosides and cyanogenic glycosides and aliphatic nitro compounds were not detected; In special pre-test on Oxytropis flavonoids, the results show that O. deflixa and O. glocialis contain mainly containing flavonoids and flavonol and two hydroxyl groups or both C3-hydroxy and C5-hydroxyl group of flavonoids.2. Studies of Determination of total flavonoids in Oxytropis plants. The results show that flavonoid content was determinated by the direct method and aluminum trichloro and aluminum nitrate staining method, in the scan of direct method and aluminum nitrite, there was not absorption peak of sample and rutin, these two methods were not representative of the wavelength of determination of total flavonoids and were not suitable methods for determination of O. deflixa and O. glocialis; while rutin was measured by aluminum trichloro staining method in the 274nm, the result indicate: precision RSD value was 1.01% and reproducibility RSD value was 1.76% and average recovery rate was 98.35% and RSD values was 0.91% and there was a better stability in the context of 120min, this method is more suitable for Determination of total flavonoids in O. deflixa.3. Studies on Ultrasonic assisted extraction of total flavonoids in Oxytropis, The results showed the optimum conditions of ultrasonic extraction were that the extraction time was 45 min and 60%using alcohol as solvent and ultrasonic power was 800Wand solid to liquid ratio was 1:10 and extraction times was 2 times, there was a significantly extraction rate and its was 19.65%.4. Determination of total flavonoids between different species of Oxytropis by Ultrasonic assisted extraction. the results indicate: the content of total flavonoids of Oxytropis plants was between 9.7 to 3.59 (mg / g) , and the content of total flavonoids from highest to lowest were: Gansu Oxytropis, O. glabra, O. deflixa, O. ochrocephala and O. glocialis.

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