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Chitin selective medium enrichment and plate culture isolated from soil samples from the North Sea mangroves biodegradable chitin to produce transparent hydrolysis circle bacteria 12 , and screened from a biodegradable transformed chitin production better aminooligosaccharide strains (HB003, the same below ) , the transformation of its biological characteristics and chitin production aminooligosaccharide fermentation experiments , the results show that : the strain colonies round , moist , pale yellow ; size is 0.5 × 1 ~ 2μm, Gram stain -negative , non-spore single polar flagella , movable ; Chief anaerobic , medium temperature , the optimal growth starting pH 7.5 to 8.5,25 ~ 35 ° C , salt-tolerant , 16s rDNA sequence analysis showed that the strains of Vibrio (Vibrio) strains of the genus similarity of 99% . 6 d, its standing fermented chitin as the sole carbon matrix liquid medium , starting pH 8.5,30 ℃ conditions can produce aminooligosaccharide 153.5 mg / ; via its production amino Oligosaccharin optimization of fermentation conditions , the test results show that : in the recipe : chitin 10g, peptone 5 g MgSO 4 · 7H 2 O 0.6g FeSO 4 0.01g, K 2 HPO 4 0.9g, KH 2 PO 4 0.6g, NaCl 20 g, distilled water 1000 mL , pH 8.0 medium , inoculum size 1% (ν / ν), fermentation production of amino Oligosaccharin , under the conditions of the fermentation temperature of 30 ° C , ventilation shake flask speed of 120 rpm output can reach 703.5 mg / L.
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