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On Plants on crop genetic improvement is important, but also an important part of the plant basic biological research. Therefore, plant salt tolerance gene cloning by the widespread concern. In recent years, with the rapid development of molecular biology, scientists have identified and cloned plant salt tolerance gene, but are very far away from the people's expectations. In this study, the room Materials salt-tolerant varieties of wheat RH8706-49, according to the information of the gene chip probe (with the amount of salt stress time expression upward), the NCBI website by electronic EST matching cloned to obtain two cDNA containing the complete ORF sequence cloned by RT-PCR, respectively, and were named TaMIP (Triticum asetivum L. Major intrinsic protein) and TaRSP (Triticum asetivum l.root specific protein). This experiment focuses on gene TaMIP, their biochemical properties were predicted by their DNAstar software, the gene encodes a protein of 300 amino acids, molecular weight of 32270.40 mw, isoelectric point of 7.43. Predicted in the NCBI website conserved domain containing two ASN-Pro-Ala (NPA) motif. By real-time quantitative PCR the detection TaMIP gene in wheat by the expression pattern of NaCl, ABA, PEG and cold stress, TaMIP gene in leaves by NaCl, ABA, PEG and cold stress showed different degrees of increase, TaMIP gene in the root by NaCl showed different degrees of increase in the ABA and PEG stress, cold stress is suppressed. Were constructed for positioning and functional studies binary expression vector TaMIP-GFP and TaMIP, for, and transformed Arabidopsis. The subcellular localization of the preliminary findings of the gene is located on the cell membrane and cytoplasm. By transgenic Arabidopsis germination rate, root length, overall plant salt tolerance. Functional studies, the gene at a certain level to improve the salt tolerance of transgenic Arabidopsis. By quantitative PCR detection of wild-type and transgenic Arabidopsis thaliana salt tolerance gene ADH1 RD29B KIN2 cor15a and P5CS1 results display The compared with control P5CS1, ADH1, RD29B, raised, is particularly obvious RD29B raised, expression of transgenic plants about 11 times that of the wild-type; the KIN2 and cor15a different degrees of reduction The transgenic Arabidopsis cor15a severely inhibited. By quantitative PCR analysis of the the the CDPK salt path FRY1, SAD1 gene FRY1 wild-type and genetically gene plant expression amount does not change, SAD1 transfer of transgenic plants with wild-type compared to a slight increase; SOS salt path SOS3, SOS2 gene SOS3 expression amount does not change SOS2 transgenic plants compared with wild-type cut. Over expression TaMIP gene effect of different signaling pathways downstream gene in Arabidopsis, speculate TaMIP gene may be the salt passage downstream terminal functional genes. By atomic absorption spectrometry to detect the Na transgenic and wild-type Arabidopsis , K , Ca 2 ion content in transgenic Arabidopsis K the , Ca 2 content higher than the wild-type and the Na ion content is less than the wild-type, in line with the mechanism of plant salt. Simultaneous detection of transgenic and wild-type Arabidopsis Pro content the transgenic Arabidopsis Pro content is higher than wild-type, in line with the mechanism of plant salt.
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