Dissertation > Excellent graduate degree dissertation topics show

Chromatin immunoprecipitation screening transcription factor AP-2 target genes

Author: HeAiLan
Tutor: ZhangJian
School: Hunan Normal University
Course: Biochemistry and Molecular Biology
Keywords: Chromatin immunoprecipitation Target gene AP-2
CLC: Q78
Type: Master's thesis
Year: 2008
Downloads: 118
Quote: 0
Read: Download Dissertation

Abstract


In the post-genomic era , DNA- protein interactions is to study the regulation of gene expression is an important area . Chromatin immunoprecipitation (chromatin immunoprecipitationassay, referred ChIP) is currently the only DNA and protein-protein interactions in vivo methods . Transcription factor stimulated by intracellular response regulate gene expression , they often pass the basic mechanisms controlling gene transcription in determining the fate of cells plays a major role. Transcription factor AP-2 involved in many mammalian development, differentiation and cancer formation transcriptional regulation of genes plays an important role. In order to identify the transcription factor AP-2 regulated downstream target genes, and ChIP experiments related conditions were optimized to obtain the optimum experimental conditions , and the use of ChIP assays in breast cancer cell line MDA-MB-453, cervical cancer HeLa cells and mouse embryos to screening and identification of transcription factor AP-2 binding sites. Through these AP-2-ChIP cell -derived DNA fragment cloning and sequencing of the discovery of new transcription factor AP-2 potential target genes in the regulation of the target genes of cancer formation, cell metabolism and other biological plays the corresponding school functions. ErbB2 gene has been reported that the transcription factor AP-2 is a downstream target genes , our experimental results are consistent with the results reported . In this study, ChIP assays were screened out transcription factor activator protein-2 (AP-2) downstream target genes for further study AP-2 functions and regulatory networks foundation.

Related Dissertations

  1. Synthesis and Performance Study of Bisphenol AP-based Polybenzoxazines,TQ320.1
  2. TLR4 Dependent Signaling Pathway in Lipopolysaccharide Induced Human Lung Carcinoma Cells Proliferation,R734.2
  3. Construction and Identification of SHH-N Gene Adeno-associated Virus Vector and Its Effection on Genes Related to Proliferation in Neural Stem Cells,R329
  4. Effections of Histone Special Sites Methylation Changes on Transcription Functions of Complete and the Second Structure Domain Flaw ELP3,Q75
  5. The Studies of Celecoxib Affecting Its Expression Profiles of microRNA Mechanisms on Human Colon Cancer Cell Line HT-29 in Vitro,R735.35
  6. Identification of Differently Expressed MicroRNA in Medulloblastoma and Function Analysis,R730.2
  7. Study on the Role of Poly(ADP-ribose)polymerase-1 in the Transcriptional Regulation of Vitamin D Receptor in Rat Thoracic Aortic Smooth Muscle Cells,R541
  8. A Preliminary Study of MiRNA Expression Profile in the Cervical Squamous Cell Carcinoma Metastasis,R737.33
  9. Protective Effects of Erythropoietin on Pancreatic Acinar Cells and Lung Tissues of Rats,R576
  10. Lithofacies Paleogeography and the Relationship with Oil and Gas Resources from Mesozoic to Cenozoic in Northern China,P618.13
  11. Promoter Characterization and Transcriptional Regulation of Human KCTD10 Gene,Q987
  12. Cloning and Identification of a Human Heart Developmental Candidate Gene KBTBD7,Q78
  13. The Downward Regulation of Rats’ OPG Expression in Steroid-induced Femoral Necrosis Rat Models and Its Mechanism,R681.8
  14. On-Line Handwritten Chinese Character Recognition Approach Based on Sentence Level,TP391.43
  15. WLAN pilot project program design and implementation,TN925.93
  16. Phosphorylation of AP-2α by the CK2 Kinase Results in Repression of AP-2α-mediated Transcriptional Activity,Q343
  17. The Effect of Neuropeptide, Soybean Flavone and C Peptide on the Expression of Nerve Cell Protein APP, NOS1 and β-secretase,Q42
  18. Some Notes on Endomorphism Rings of Quasi AP (AGP)-Injective Modules,O153.3
  19. LMP-1 to Human Nasopharyngeal Carcinoma Cell Line CNE1 MiRNA Expression Spectrum Influence,R739.63
  20. Association of AP-4 Expression in Regional Lymph Nodes with Recurrence in Colorectal Cancer,R735.3
  21. Detection of AP-1 Binding to CyclinD1 and Caspase3 Gene Promoter in A549 Cells with Chromatin Immunoprecipitation,R734.2

CLC: > Biological Sciences > Molecular Biology > Genetic engineering (genetic engineering)
© 2012 www.DissertationTopic.Net  Mobile