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During seed development in different genotypes of bitter gourd varieties of fruits , seeds external morphological and physiological indicators of change and hypoglycemic polypeptide -K extraction, purification conditions , to determine the best seed harvest time . The results are as follows: 1 cluster analysis of 28 types of plants , fruits and seeds of 33 bitter gourd germplasm resources morphological traits tested resources be divided into three groups : the wild-type group ( group Ⅰ ) , the dense tumor small fruit type group ( group II ) and grew up in the fruit type group ( group III ) ; various types of groups there are obvious differences in morphology , can reflect the typical morphological characteristics of the group , to reflect their genotype there are obvious differences . There are significant regional differences among groups within the geographical scope of the genetic short distance , while the genetic distance between regions . 2 , balsam pear varieties during seed development in wild-type , grew up fruit , dense the tumor small fruit type 3 groups of 12 morphological traits of fruits and seeds of change and seed viability , dry weight , and water content changes conducted a survey , the seed development process is divided into three periods: the rapid enlargement stage , dry matter accumulation stages of stage and mature dehydration of ; genotypes of bitter gourd seeds in regular changes occurred during the development of the wild-type bitter gourd the MC4 seed development relatively short , developed to enrich and mature in 18 days after pollination (18DAA) ; grew up in the fruit - MC6 bitter gourd seed development time is relatively long , 22 days after pollination (22DAA) developmental enrichment mature ; the dense tumor small fruit - MC11 bitter gourd seed development the longest time , that 22DAA developmental enrichment mature external morphology during the development of fruits, seeds and seed dry matter content , water content are regular changes occurred , there is a significant genotypic differences . 3 , by acid , alkali solution extraction precipitate , TLC preliminary identification of 0.29 is very similar to the reported hypoglycemic polypeptide -K migration rate of 0.288 , and Khanna academicians , initially identified in Figure 4-3 point B protein may hypoglycemic polypeptide - K . 4, the optimization of conditions hypoglycemic polypeptide -K denaturing polyacrylamide gel (SDS-PAGE) , the concentration of the separation gel is 12% , the degree of crosslinking of 3% denaturing polyacrylamide gels can be the target protein band good separately.
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