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In this experiment, the diploid spend high generation seed material, establish the large flower high generation vitro rapid propagation system, to study the large flowers of high-generation tissue culture mutagenic effects, filter out the good variation of plants, rich germplasm resources, ornamental value, introduction and breeding research for the flowers to open up new ways for a try. The test results are as follows: 1, in the MS (the control), MS ZT 0.5 , MS of TDZ 0.5 , MS 2,4-D 0.5 , the MS NAA 0.5 five kinds of medium MS ZT 0.5 MS thidiazuron (TDZ) 0.5 both callus induction, explants body not only produces a callus and adventitious buds differentiation. MS ZT 0.5 adventitious bud differentiation rate of up to 83%, in this trial, MS ZT 0.5 optimal medium for the big flower on behalf callus and adventitious bud induction , 1/2MS IBA 2 as a big flower on behalf of plantlets optimum rooting medium. 2 to 0Gy, 10Gy, 50Gy, 100Gy, 150Gy and 200Gy different doses 60 Coγ ray radiation seeds to study the growth and impact of a big flower on behalf of plantlets results show that, in the 50-200Gy range of root and leaf growth and plant height are affected to varying degrees inhibition, and the inhibition of the extent of the radiation dose was positively correlated with 10Gy can promote the growth of roots and leaves. As the dose increased, mortality, tissue culture growth the 41d when half lethal dose (LD 50 ) for 150Gy was recommended as. The plantlets cultured 70d each treatment, a small amount of bottle flowering pattern, color and petal variation. These results suggest that 60 The Coγ ray radiation can increase the mutation frequency and mutagenic effect obviously favor the big flower generation breeding colorful flowers, beautiful plant type variation type. 3, were soaked with different concentrations of colchicine big flower on behalf of germinating seeds 6h, 24h, with the improvement of colchicine concentrations, the greater the damage to tissue culture by the higher mortality rate, low concentrations of long processing hurt more than the high concentration of short-term processing, showed the same effect in the the plantlets growing and profusion bud differentiation. This trial, colchicine soak the a 6h plants victimization smaller induced double plants, and in a concentration of 0.4%, the doubling rate of up to 42%. Doubling Plant leaves hypertrophy color deepened, thick stem section shorter, the leaves and stems hairy growth potential in the form of performance; showed a pattern of variation and color in the flowers and colors deepened. Using BD FACS Calibur flow cytometer Ploidy identification: big flower variant plants DNA content distribution, diploid and tetraploid chimera.
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