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Tissue Culture and Induction of Polyploidy with Colchicine in Scutellaria Barbata D.Don

Author: ZuoDaZuo
Tutor: ChenChongShun
School: Nanjing Normal University
Course: Botany
Keywords: Medicinal Plants Banzhilian Tissue culture Rapid propagation in vitro Polyploid Induction Colchicine
CLC: S567.239
Type: Master's thesis
Year: 2007
Downloads: 353
Quote: 1
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Abstract

Medicinal plant Scutellaria barbata (Scutellaria barbata D.Don) is an application of a wide range, use large and pharmacodynamic features traditional Chinese medicine, in recent years, studies have shown that also has a good anti-tumor effect. Induced polyploidy can improve the uncommon of medicinal herbal medicine ingredients reported, this approach is feasible. In this theoretical basis, first established Scutellaria barbata vitro micropropagation system colchicine treatment shoot tips induced banzhilian polyploid plants and were identified, the final determination of the polyploid strains have been identified medicinal component of total flavonoids. The shoot tips of the Scutellaria barbata potted seedlings for the starting material, containing 2% available chlorine NaClO solution disinfection 5min growth for aseptic seedlings transferred to MS medium. Take the sterile seedlings 1cm long shoot tips were cut, 0.8cm long stem segments with axillary buds and axillary buds without stem segment explants for tissue culture. Scutellaria barbata vitro micropropagation system established: 1cm shoot tip explants induced multiple shoots with hormone 6-BA and IBA combination MS 6-BA 0.5mg · L ^{-1 IBA 0.1 mg · L ^{-1 most clustered buds induced proliferation coefficient of 6.5; 1cm shoot tip explants induced multiple shoots with hormone 6-BA and NAA, MS 6-BA 0.5 mg · L ^{-1 NAA 0mg · L ^{-1 the clustered buds induced proliferation coefficient up 6.1; 0.8cm with axillary stem segments as explants, MS 6-BA 0.5 mg · L ^{-1 NAA 0mg · L ^{-1 most clustered buds induced proliferation factor of 5.7; outside 0.8cm stem segments with axillary The explants were placed across the medium, MS 6-BA 0.5 mg · L ^{-1 NAA 0mg · L ^{-1 clustered buds induced maximum proliferation coefficient of 4.8. Therefore best medium for the induction of multiple shoots MS 6-BA 0.5mg · L ^{-1 IBA 0.1mg · L ^{-1 . The Scutellaria barbata callus induction: the leaves and stems as explants induced callus on hormone 6-BA and NAA medium, without success. Of Scutellaria barbata polyploid induction of identification: 0.2% colchicine soak the shoot tips 3h induced polyploidy the largest induction rate up to 10%, the survival rate was 25%. From the Scutellaria barbata morphological characteristics and cytology of control and polyploid comparison, the four lines were identified by flow cytometry, are polyploid chimeric. Determination of the three of Scutellaria barbata polyploid strains of medicinal ingredients total flavonoids content, respectively, is 1.88%, 1.85% and 1.69%, 1.67% of the untreated control strains; recoveries 99.1% and the relative standard deviation of 3.56%.}}}}}}}}}}

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