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Research on the Making Process of the Compound Tissue-engineering Scaffolds of the PLA/silk Fibroin and Biological Evaluation

Author: XingShuai
Tutor: XiaYaYi
School: Lanzhou University
Course: Surgery
Keywords: Polylactide / silk fibroin Biological Evaluation Tissue Engineering
CLC: R318.08
Type: Master's thesis
Year: 2008
Downloads: 99
Quote: 2
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Abstract


Objective: To study the poly lactic acid ( PLA ) / silk fibroin composite tissue engineering scaffolds alone polylactic acid stent compared to evaluate the biological performance . Methods: the silk fibroin powder was added to the 40% calcium chloride solution was heated and stirred 60min , made ??of silk fibroin , calcium chloride solution , and the solution was poured into dialysis bags and dialyzed in the sink 72h , to obtain silk fibroin aqueous solution. On silk fibroin aqueous solution of polylactic acid scaffold soaked , dried polylactide / silk fibroin composite scaffolds prepared . Two stents divided into the PLA stent group and composite stent group , according to the ISO-10993 standard for hemolysis experiments , dynamic clotting time experiment , cytotoxicity assays , stimulation experiments and pyrogen experimental and comparison itemized . Results: The two materials in the stimulation experiments did not cause the animal skin significantly stimulate the standards ; in pyrogen experiment , two scaffolds induces elevated body temperature total degree in 0.2 ℃ and the body temperature rises 1.0 ℃ no significant difference ; the hemolytic experiments , the two stents materials samples hemolytic rate of less than 5% (P = 0.000), showed that the composite scaffold anti- hemolytic than PLA bracket ; dynamic clotting time experimental composite scaffold dynamic coagulation time of 37min , the PLA stent dynamic clotting time for 26min , the anticoagulant properties of the composite scaffold significantly better than the PLA stent ; the cytotoxicity experiment in the the composite stent group cell growth significantly better than the the PLA stent group , composite scaffolds cytotoxicity significantly less than the PLA bracket . Conclusion : polylactide / silk fibroin composite scaffold with better biocompatibility than pure polylactic acid scaffolds can be used as scaffolds implanted .

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CLC: > Medicine, health > Basic Medical > Medical science in general > Biomedical Engineering > General issues > Biomaterial
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