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Objective To observe the expression and distribution of AQP1 and AQP4 during development of normal rat cornea and lens.In order to confirm the emergence,location and tendency of AQP1 and AQP4 in rat cornea and lens.Methods cornea and lens of eyes dissected from embryonic day (E)13,E15,E18,and E20,postnatal day(P)0,P7,and P14,and postnatal week 8 rats were subjected to Immunohistochemical technique with AQP isoform-specific antibodies.Resuits Developmentally,AQP1 was detected as early as E15 in immature corneal endothelium and lens epithelial cells.The period of E18 expressed enhancer.The period from E20 to P14 and adult expressed steadily in the rat development.However,corneal epithelial cells did not expressed.In contrast,AQP4 was first observed at P7 in the developing cornea and len.It expressed strong in P14 and expression persisted throughout development up to adulthood.However,AQP4 did not expressed in corneal endothelium.Conclusion The emergence,location and tendency of AQP1 are different from AQP4.expression of AQP1 is dynamic along with cornea and lens development.These two proteins are important to keep physiology balance and transparency of cornea and lens.
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