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Objective: laminin extracellular matrix (extracellular matrix, ECM) of the key ingredients . 67 kD laminin receptor (67 kD laminin receptor, 67LR) is a non- laminin integrin receptor , and laminin interactions regulate tumor proliferation, adhesion , angiogenesis , invasion and metastasis of tumor . Our previous findings 67LR high expression can promote liver cancer cell invasion , this study aimed to explore 67LR on metastasis-associated gene expression , to clarify 67LR promote the molecular mechanism of invasion and metastasis of HCC . Methods: Transient transfection and stable cell lines constructed to achieve high expression of 67LR , 67LR using RNA interference technology to reduce the expression of the inhibitory effect using flow cytometry and semi-quantitative RT-PCR analysis ; semi- quantitative RT-PCR using technical analysis 67LR effects on gene expression , some changes in gene expression by fluorescence quantitative PCR validation ; MMPs activity in gelatin zymography through determination . Results : 67LR high expression of stable cell lines LR4, its MMP-2, MMP-9 expression lower than 67LR expression LR6 and the control group pcDNA-1 cells were significantly increased, gelatin zymography also revealed , LR4 cells secrete MMP -2 and MMP-9 activity was significantly increased ; 67LR transiently transfected with the recombinant plasmid Huh-7 cells, MMP-2, MMP-9, TIMP-1, TIMP-4, Hpa, uPA, Cath-B and Cath- D expression was significantly increased, while TIMP-2, TIMP-3 expression was significantly decreased ; in SMMC-7721 cells and Huh-7 cells , 67LR downregulation can inhibit MMP-2, MMP-9, TIMP-1, TIMP-4, Hpa, uPA, Cath-B, Cath-D gene expression , etc. , to promote TIMP-2, TIMP-3 expression . Conclusion : 67LR can be adjusted by MMP-2, MMP-9, TIMP-1, TIMP-4, Hpa, uPA, Cath-B, Cath-D, TIMP-2, TIMP-3 and other genes , thereby promoting liver cancer in vitro invasion .
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