|
Purpose of the mRNA and protein levels, testing APEX1 and HBV markers in HCC (hepatocellular carcinoma, HCC) expression, and to explore synergies APEX1 expressing HBV markers with invasion and metastasis of HCC and its significance. At the same time, by detecting 100 cases of HCC in Fujian Province, Fujian patients and 100 normal population APEX1 single nucleotide polymorphisms, to explore its correlation with liver cancer. Methods tissue microarray, immunohistochemistry and in situ hybridization techniques were used to detect 130 cases of HCC, 96 cases of adjacent liver tissue, 46 cases of distal cancerous liver tissues and 29 cases of extrahepatic metastases APEX1 protein, HBsAg, HBcAg, APEX1mRNA and HBV DNA expression, and its relationship with clinicopathological parameters and its significance; application ligase detection reaction (ligase detection reaction, LDR) detects APEX1 Ile64Val, Asp148Glu and Gly241Arg 3 single nucleotide polymorphisms point and for linkage disequilibrium and haplotype analysis to explore the three SNPs in Fujian HCC population distribution, and its relationship with liver cancer risk in this population and its significance. Results 1 immunohistochemistry and in situ hybridization showed that, APEX1 in HCC, paraneoplastic, far cancerous liver tissues and normal liver tissue expression levels gradually decreased, the difference was significant (P lt; 0.001); in HCC, With the increase of pathological grade, APEX1 expression gradually increased (P lt; 0.05), compared with tumors without metastasis, tumor metastasis group APEX1 expression was significantly increased, and the tumor capsule group APEX1 envelope protein expression compared with non- infiltration was significantly higher (P lt; 0.0001). APEX1 joint nuclear cytoplasmic protein expression ratio in adjacent liver tissue, far cancerous liver tissue, HCC and metastases group in ascending order, the difference was significant (P = 0.001). 2. HBsAg and HBV DNA in the far-cancer group and the adjacent group was significantly higher than HCC group and metastasis group, the difference was significant (P lt; 0.001). HBcAg in adjacent liver tissue, far cancerous liver tissues slightly HCC, but the difference was not significant (P gt; 0.05). In addition to expression of HBsAg negative correlation with APEX1mRNA, the difference was statistically significant (P = 0.014), the rest were among the indexes unrelated (P gt; 0.05). 3 ligase detection reaction showed, Gly241Arg polymorphism and the incidence of hepatocellular carcinoma is closely related (P = 0.002), while Ile64Val and Asp148Glu polymorphisms with HCC unrelated (P gt; 0.05), Asp148Glu and Gly241Arg locus linkage disequilibrium exists between (D '= 0.519). Conclusions: 1. APEX1 with HCC malignant phenotype closely related. APEX1 cytoplasmic protein expression emergence may be associated with tumor invasion and metastasis. APEX1 overexpression and cytoplasmic protein expression may be associated with HCC prognosis; 2. APEX1 expression in HCC patients age, sex, tumor size, histological type and other clinicopathological parameters are irrelevant; 3. HBsAg and HBV DNA in HCC group The expression was significantly lower than the distant cancer group and the adjacent group; 4. APEX1 Gly241Arg polymorphic loci carrying GA genotype populations may be more susceptible to liver cancer, APEX1 Gly241Arg polymorphism screening can be used as a risk factor for hepatocellular carcinoma predilection ; 5. Ile64Val and Asp148Glu polymorphisms with HCC unrelated.
|