Dissertation > Excellent graduate degree dissertation topics show

The Expression of SASH1 in Human Gastric Cancer Tissue and the Research on the Gene SASH1 in GES-1 Cell Line by RNAi

Author: ChenZhiFen
Tutor: GuanGuoXian；LuHuiShan
School: Fujian Medical
Course: Oncology
Keywords: SASH1 gene Gastric cancer RNA interference BCL-2 gene
CLC: R735.2
Type: Master's thesis
Year: 2008
Downloads: 57
Quote: 0
Read: Download Dissertation

Abstract


The Objective : SASH1 the gene (SAM-and SH3-domain containing 1) is a newly discovered candidate tumor suppressor gene , the research SASH1 protein expression in gastric cancer tissues and build SASH1 small interfering RNA expression vector inhibit SASH1 gene in GES-1 cells in the expression . Method: 1 , collected 94 cases of resected tissue and 15 cases of paraneoplastic tissue, by immunohistochemistry the method detection SASH1 protein expression , and analysis of the relationship between the SASH1 expression with clinical and pathological features of gastric cancer ; 2 design SASH1 gene RNAi interference target and pSilencer 1.0- U6 plasmid construct 2 SASH1 small interfering RNA expression vectors were named as pSilencer-1.0-U6-SASH1-1 and into pSilencer -1.0 - U6 - SASH1 -2 ; through LipofectamineTM2000 the recombinant the plasmid was transfected into gastric epithelial cell GES-1 , compared with four MTT colorimetric (MTT) recombinant plasmid after transfection GES - 1 cell proliferation ability to change with immunocytochemistry , RT- before and after transfection SASH1 PCR assay gene expression changes GES-1 cells BCL-2 gene expression changes were compared before and after transfection . Results: 1 , gastric carcinoma SASH1 protein expression was significantly lower than the adjacent tissues , the difference was statistically significant ( P = 0.001 ) ; SASH1 expression and depth of invasion in gastric cancer tissues (P = 0.001), lymph node metastasis ( P lt; 0.001), related to the degree of differentiation (P = 0.021) and TNM stage (P = 0.047) , and has nothing to do with age (P = 0.513) and gender (P = 0.720) ; 2 , transfected with the recombinant plasmid pSilencer-1.0 -U6-SASH1-1 group GES-1 cells 72h , 96h, 120h using the MTT assay measured the proliferative capacity than the control group and pSilencer - 1.0 - U6 - SASH1 - 2 experimental group ( P lt; 0.05 ) ; transfected with the recombinant plasmid pSilencer- 1.0 -U6 - SASH1 - 1 group GES-1 the intracellular SASH1 gene mRNA and protein expression levels of BCL -2 gene expression levels . Conclusions : 1 level of SASH1 protein expression in gastric cancer clinical pathological features closely related ; 2 , recombinant plasmid pSilencer - 1.0 - U6 - SASH1 - 1 can effectively inhibit GES-1 cells within the SASH1 of gene expression ; 3 , the application of RNAi inhibits GES- gene expression after 1 cells SASH1 promote the proliferation of GES-1 cells ; 4 , inhibition of GES-1 cells within the SASH1 of gene expression after GES-1 cells within the BCL-2 expression levels , may prompt the SASH1 downregulated BCL-2 play a series of biological effects .

Related Dissertations

  1. The Effects of Pu-erh Tea on Prevetion and Treatment of Tumor,R285.5
  2. Study of Oridonin on SGC-7901 Cell Proliferation by Inhibitting Cell Cycle Proteins,R285
  3. Expression Dynamics of Pheromone Binding Proteins and Expression Influences by Mating and Knockdown of Cryl in Spodoptera Exigua,S433.4
  4. Molecular Cloning, Mrna Expression and Rnai of Nadph-Cytochrome P450 Reductase Gene in Helicoverpa Armigera (Hǘbner),S435.622.3
  5. Effects of BMPR-IB Gene Silencing by Small Interfering RNA on Apoptosis of Porcine Pollicular Granulosa Cells and Ecpression of BMP Pathy-Way-Ralted Genes,S828
  6. Inhibition of Myostatin (MSTN) Expresstion in Primary Porcine Fetal Fibroblasts by Lentivector-mediated RNAi,S828
  7. Yu Ren- Cun Academic Thought and clinical experience in the clinical observation of combination chemotherapy in advanced gastric cancer with Yiqihuoxuejiedu side,R249
  8. A Preliminary Study on the Regulation of Hepatoma Cell Biological Behavior by MCFP,R735.7
  9. The DNA Cloning and RNA Interference of Some Functional Genes in Laodelphax Striatellus (Fallen),S435.112.3
  10. Expression and Clinical Significance of PTEN and Caspase-7 in Gastric Cancer and Precancerous Lesion,R735.2
  11. Adhesion Molecule P-selectin Expression in Gastric Cancer Peritoneal Metastasis,R735.2
  12. The Effect of RNA Interference-mediated ERCC1 Gene on the Chemo-treatment Sensitivity of NSCLC in Vitro,R734.2
  13. The Contribution of the CCl22, CCR4 in the Milky Spots Peritoneal -metastasis of Gastric Cancer,R735.2
  14. The Study of Inhibtion Effect by shRNA Expression Vector on Herpes Simplex Virus Type 2 UL54,R346
  15. Protein Expressions of EGFR、K-ras、P53 and Ki-67 in Colorectal Cancer, Gastric Cancer and Esophageal Cancer,R735
  16. Variation of Immune System and Clinical Experiment Indicators in Patients with Gastric Cance and Its Association with 15 STR Loci,R735.2
  17. Research of Expression and Clinical Significance of p53、PCNA and PTEN in Gastric Cancer,R735.2
  18. Expression of Chemokine Receptor CXCR1, CXCR2, CCR7 in Peritoneal Metastasis in Gastric Carcinoma and Its Significance,R735.2
  19. The Influence of RNA Interference Suppression EphB4 Expression for the PANC-1 Cells of Pancreatic Cancer,R735.9
  20. The Regulation and Significance of NHE1 in Acidic Micro-environment of Gastric Carcinoma,R735.2
  21. Establishment and Study of the Cultured Model of Microencapsulated Human Gastric Carcinoma Cells,R735.2

CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Gastric neoplasms
© 2012 www.DissertationTopic.Net  Mobile