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Relationship between FOLR1 and Paclitaxel-resistance in Nasopharyngeal Carcinoma

Author: ShiXiaoJing
Tutor: TanGuoLin
School: Central South University
Course: Otolaryngology Head and Neck Surgery
Keywords: Nasopharyngeal carcinoma Paclitaxel Resistant cell lines FOLR1 gene expression
CLC: R739.63
Type: Master's thesis
Year: 2008
Downloads: 73
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Abstract


Objective To investigate the mechanism of nasopharyngeal resistance to chemotherapeutic drugs, the study attempts to establish human nasopharyngeal carcinoma cells to paclitaxel (Taxel) resistant cell lines HNE-2/Taxol, and to explore FOLR1 with nasopharyngeal carcinoma cell sensitivity to paclitaxel and resistance relationship; Method 1. inducer with paclitaxel in vitro with high-dose medication and gradually increase the dose effect of a combination of methods in human nasopharyngeal carcinoma cell line HNE-2 cells, trying to build resistance to paclitaxel nasopharyngeal cells HNE-2/Taxol; 2. using colony formation assay (colony formation assay) were detected nasopharyngeal carcinoma cell line CNE-2, CNE-1, HNE-2 and drug sensitivity of cells to paclitaxel (IC 50 ), and determine the resistant cell line resistance index (RI); 3. using semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) in nasopharyngeal measured FOLR1 parental cell line CNE -1, CNE-2, HNE-2 and its resistant cell line expression levels. Results 1. Successfully established resistant cell lines resistant to paclitaxel HNE-2/Taxol, for the study of paclitaxel resistance mechanisms provide an experimental model; Dividing established nasopharyngeal carcinoma cell line resistant HNE-2/T5 and HNE -2/T10 colony formation assay performed three times to determine its IC 50 values ??were 1.70 ng / ml and 5.23ng/ml, resistance index were 1.42 ± 0.15,4.36 ± 0.21; 2. nasopharyngeal carcinoma parental cell line CNE-2, CNE-1, HNE-2 half inhibitory amount (IC 50 ) were 5.02 ng / ml, 0.86ng/ml, 1.20ng/ml; 3. FOLR1 RNA levels detected from the nasopharyngeal carcinoma cell line CNE-1, CNE-2, HNE-2 expression was found FOLR1 expression and cancer cell sensitivity to paclitaxel was negatively correlated R 2 = 0.9923 (p <0.05); 4. semi-quantitative RT-PCR analysis FOLR1 in nasopharyngeal HNE-2/T5 and HNE-2/T10 resistant cell lines expressing, expression and nasopharyngeal carcinoma found FOLR1 resistant drug resistance index of cells was positively correlated with the correlation coefficient R 2 = 0.9944 (P <0.05). Conclusions 1. HNE-2/Taxol established in this experiment is one pair of paclitaxel-resistant human nasopharyngeal carcinoma drug-resistant cell lines to study the clinical course of chemotherapy nasopharyngeal acquired resistance and the mechanism of resistance to paclitaxel important experimental model; 2.FOLR1 genes in wild-type parental nasopharyngeal carcinoma cell line CNE-1, CNE-2, HNE-2 expression levels in their sensitivity to paclitaxel was negatively correlated (p <0.05); 3.FOLR1 resistant gene and nasopharyngeal carcinoma cell line resistant to paclitaxel HNE-2/Taxol resistant phenotype was positively correlated (p <0.05); 4.FOLR1 genes may be reversed and nasopharyngeal carcinoma cells resistant to paclitaxel drug target molecule for cancer therapy .

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CLC: > Medicine, health > Oncology > Department of Otolaryngology tumor > Pharyngeal tumors
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