|
Objective: To detect aquaporin 1 (Aquaporin 1, AQP1), vascular endothelial growth factor (VascularEndothelial Growth Factor, VEGF) and microvessel density (Microvessel Density, MVD) in 60 patients with laryngeal squamous cell carcinoma (laryngeal), 20 cases of adjacent tissues (paraneoplastic group) and 17 cases of vocal cord polyp (vocal cord polyps) expression of the difference between the three-factor correlation to explore the significance of AQP1 in laryngeal squamous cell cancer and the relationship with angiogenesis consider the AQP1 whether as squamous cell carcinoma of the larynx markers for early diagnosis and to determine the clinical classification and staging and pathological grading and laryngeal whether the correlation observed in lymph node metastasis and without lymph node metastasis The difference is statistically significant, the treatment plan to guide the choice of operative methods and postoperative whether to give adjuvant treatment such as radiotherapy and chemotherapy. Pathogenesis of laryngeal squamous cell carcinoma is the future research to provide the scientific basis for guiding the diagnosis and prognosis of laryngeal squamous cell carcinoma. Methods: Using immunohistochemical methods to detect each group organization the AQP1, VEGF protein expression, measured microvascular endothelial cells marked by CD34 in laryngeal squamous cell carcinoma, carcinoma paraneoplastic vocal cord polyp microvascular density (MVD) into sample cases clinical classification, clinical stage and pathological grade laryngeal cancer at the Association for International Cancer (UICC) TNM Classification Standard (1997), the data obtained using analysis of variance, chi-square test, rank sum test and Spearman rank correlation statistics processing, The application SPSS13 software for statistical analysis. The results: of AQP1, VEGF and CD34 in different degrees of expression in laryngeal carcinoma paraneoplastic group and vocal cord polyp group, the significant difference between the three groups significant (P <0.05) between the two groups of AQP1 in cancer and paraneoplastic adjacent noncancerous polyps comparison was statistically significant (P <0.01), but was not statistically significant (P> 0.05) between laryngeal carcinoma and vocal polyps group AQP1 Description AQP1 and vocal cord polyps closely related to edema; AQP1 expression in laryngeal squamous cell carcinoma clinical stage (P <0.01), lymph node metastasis (P <0.01), pathological grade and gender (P <0.05) were related, but the clinical classification and Age no correlation (P> 0.05); VEGF expression in laryngeal squamous cell carcinoma clinical stage (P <0.01), lymph node metastasis (P <0.01), and gender (P <0.05), but with clinical classification had no correlation with age, and pathological grade (P> 0.05); clinical stage of CD34 expression in laryngeal squamous cell carcinoma (P <0.01), clinical classification (P <0.01), and lymph node metastasis (P <0.05) were related , of AQP1 and VEGF, AQP1 and CD34 and CD34 and VEGF expression in laryngeal squamous cell carcinoma but with sex, age and pathological grade (P> 0.05) correlation; positive correlation (P <0.01). Conclusion: The high expression of AQP1, VEGF and CD34 in laryngeal squamous cell carcinoma and TNM clinical stage, sub-type, histological grade and lymph node metastasis of prompted AQP1 tumor vascular permeability increase promote the rapid transport of water in the tumor cells, promoting tumor angiogenesis, thereby promoting tumor growth and metastasis, and upregulation of the protein in laryngeal squamous cell carcinoma, suggesting that the protein in laryngeal carcinoma, play an important role in the development of . In short, AQP1 in laryngeal squamous cell carcinoma occurs with promoting cancer growth, metastasis and angiogenesis role in the development process; AQP1 in-depth study in favor of future studies of the pathogenesis of laryngeal squamous cell carcinoma, the guidance of the diagnosis and treatment of laryngeal cancer and prognosis.
|