Dissertation > Excellent graduate degree dissertation topics show

Effects of NGF on the Neural Cells and Egr-1 Gene Expression in Patients with Epilepsy

Author: FangQiong
Tutor: ChenYanHui
School: Fujian Medical
Course: Pediatrics
Keywords: Epilepsy Nerve Growth Factor Early growth response gene 1 Hippocampal Nerve cells People
CLC: R742.1
Type: Master's thesis
Year: 2008
Downloads: 68
Quote: 0
Read: Download Dissertation


[Objective] To understand the nerve growth factor in the cerebrospinal fluid of patients with epilepsy (nerve growth factor, NGF) level of different concentrations of NGF on human epileptic hippocampal CA1 region of the total number of nerve cells, astrocytes, the number of neuronal survival impact different concentrations of NGF of nerve cells Egr-1mRNA expression to explore NGF on the role and mechanisms of epileptic brain damage nerve cells. [Methods] ELISA (enzyme-linked immunosorbent assay, ELISA) were measured epilepsy group (n = 28) and non-epileptic control group (n = 21) in cerebrospinal fluid NGF levels; using dissociation cell instantly culture techniques people with epilepsy epileptiform discharges after inspection originated in the temporal lobe or hippocampus and pathological examination found no specific pathological changes in the nerve cells of the hippocampal CA1 (n = 16) were cultured, and the establishment of concentration were 12.5, 50, 100 ng / ml the NGF plus drug group and without NGF blank control group, after 4h incubation, nuclear staining by immunofluorescence cytochemistry double benzene imide (Bisbenzimide, Bb), joint cell-specific marker proteins GFAP, MAP2 mark Star shaped glial cells, neurons, and count the total number of nerve cells survive, and astrocytes and neurons number and calculation of astrocytes and nerve elements of the total percentage of nerve cells, while taking advantage of the RT-PCR technique half different concentrations of NGF quantitative analysis of the impact of early growth response gene -1 (early growth response gene-1, Egr-1) mRNA expression in nerve cells. [Results] 1. Cerebrospinal fluid in NGF content epilepsy group and non-epileptic control group, respectively (27.31 ± 6.36) pg / ml, (24.09 ± 4.96) pg / ml, NGF levels in the cerebrospinal fluid groups no significant difference (P gt; 0.05 ). 2 in NGF concentration 12.5,50,100 ng / ml, the number of nerve cells in the hippocampal CA1 region were (82.02 ± 11.08), (104.71 ± 14.24), (127.11 ± 14.35) / field of view, and without NGF blank The control group (67.71 ± 9.21) / field compared, the difference was statistically significant (P lt; 0.01), total number of neuronal survival with NGF concentration increased and increased with the NGF dose was the dose-effect relationship (P lt; 0.01). NGF concentration 0,12.5,50,100 ng / ml, the number of astrocyte GFAP () were (23.11 ± 4.39), (30.73 ± 5.16), (44.25 ± 6.76), (59.57 ± 6.23) / vision; MAP (2) the number of neurons, respectively (8.82 ± 1.74), (14.81 ± 2.87), (22.51 ± 3.10), (32.17 ± 2.52) / vision, astrocytes and nerve neuron survival number of NGF-dose dose-effect relationship, with increased NGF concentration increased (P lt; 0.05). With the NGF concentration increased astrocytes and neurons accounts for the percentage of nerve cells was increased, showing the same trend of increasing. The NGF concentration 0,12.5,50,100 ng / ml, GFAP () astrocytes and MAP2 () neurons representing the percentage of nerve cells in the median (interquartile range) 34.94 (31.16 to 36.91)%, 38.49 (34.70 to 39.94)%, 42.36 (40.22 to 45.13)%, 45.92 (43.79 to 51.53)% and 13.01 (10.65 to 15.55)%, 18.35 (16.37 to 19.92)%, 21.94 (21.36 to 22.84) 25.33 (23.98 to 26.84)%; comparing different concentrations of NGF GFAP () astrocytes and MAP2 () neurons account for the impact of nerve cells percentage change found no significant difference significance (P gt; 0.05). When NGF concentration, as 0,12.5,50,100 ng / ml, the nerve cell expression of Egr-1mRNA AEgr-1/Aβ-actin (0.46 ± 0.14), (0.63 ± 0.14), (0.80 ± 0.15) , (1.12 ± 0.12), the treatment group were not added NGF control group comparison, the existence of significant forward difference (P lt; 0.01), the same nerve cells in the expression of Egr-1mRNA amount with NGF dose showed a dose-response relationship, with the NGF concentration increased and expression was increased (P lt; 0.01). And in the NGF effect, the number of nerve cells and the corresponding expression of Egr-1mRNA the amount of a positive correlation (r = 0.780, P lt; 0.01). [Conclusion] The results showed that refractory epilepsy in cerebrospinal fluid NGF levels than non-epileptic control group no significant increase; NGF can improve in vitro cultured human epileptic brain hippocampal CA1 (12.5 to 100) ng / ml range the number of cells and GFAP () astrocytes and MAP2 () the number of neurons, the role of NGF concentration increased was positively correlated, suggesting that NGF has a protective effect on nerve cells; comparing different concentrations of NGF star enhance the role of glial cells and neurons survival showed no significant differences, suggesting that NGF promote astrocytes and neurons in hippocampal CA1 region of people with epilepsy have a protective effect on nerve cells; NGF expression of Egr-1mRNA role, the increase in the amount of the expression of NGF concentration was a positive dose-effect relationship, suggesting that Egr-1 may play an important role in the NGF-mediated nerve cell protection.

Related Dissertations

  1. Investigation of Sports for Elder People and Countermeasure of Development in Fuzhou City,G812.48
  2. The Flowing People’s Children Learning Adaptability and with Academic Self-efficacy and Mental Health Relationship,G441
  3. The Construction of Multiple Administrative Accountability Authorized by the People to the Governments and Officials in China,D630
  4. Humanism in Environmental Reporting,G212
  5. Diffusion Tensor Imaging Study of Idiopathic Epilepsy at 3.0T High Field Magnetic Resonance Scaner,R445.2
  6. Yunnan Nationalities Region Compulsory Education Quality Evaluation,G522.3
  7. The Measurement and Application of Communicating Vein in Lower Limbs Adopting Color Doppler Ultrasound,R445.1
  8. Influence of Estrogen and H.pylori on Human Duodenal Mucosal Bicarbonate Transport Protein,R573.1
  9. Apply the EEG-fMRI to Explore the Epilepsy with Continuous Spike-waves During Slow Sleep,R742.1
  10. Uncertainty and metafiction : \,I712.074
  11. Post-treatment on ischemia-reperfusion injury in rat lung EGR-1 expression,R363
  12. The "people-oriented" Concept in Gansu Province Urbanization Construction under the New Research,F299.27
  13. In Vitro Study of Theeffects of Fullerol on the Hippocampal Synaptic Plastisity,R741
  14. Research Report on People’s Mediation in Anhui Province,D926
  15. Research about Cognitive Model of Rumors Based o Hopfield Neural Network,B842.1
  16. The Prevalence and Risk Factors of Non-fatal Injury Among Older Adults,R181.3
  17. Harbin City People's Mediation System empirical research,D926
  18. Jinan City, the elderly and cultural life of sports and leisure visits,G812.4
  19. Expo Cultural scenario \,TS941.2
  20. Shanghai Preliminary study on elderly housing update mode,TU241.93
  21. Roles of Small Conductance Calcium-activated Potassium Channels in Mice with Alzheimer’s Disease,R749.16

CLC: > Medicine, health > Neurology and psychiatry > Neurology > Brain diseases > Epilepsy
© 2012 www.DissertationTopic.Net  Mobile