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The Experimental Study on Plasm Endotoxin, TNF-α, IL-6 and Expressions of Hepatic COX-2 after Intestinal Firearm Wound

Author: LvYueTao
Tutor: FengDeYuan；ZhangYongJiu；LiuJiangWei
School: Shihezi University
Course: Surgery
Keywords: Endotoxin Tumor necrosis factor -α Interleukin-6 Cyclooxygenase-2 Firearm injuries Liver injury
CLC: R656.7
Type: Master's thesis
Year: 2008
Downloads: 22
Quote: 0
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Abstract


Objective: In this study, through the establishment of the pig abdomen intestinal firearm wound model, observed the change of the structure and function of liver injury after injury plasma endotoxin, TNF-α, IL-6 levels in liver tissue expression of COX-2 dynamic change of plasma endotoxin and TNF-α, the relationship between COX-2, IL-6 levels in liver tissue after intestinal firearm wound secondary liver injury in the role, aimed at secondary liver injury after abdominal firearm wound prevention and treatment of the theoretical and experimental basis. Methods: 42 healthy Landrace pigs, were randomly divided into 7 groups: control group and to abdominal firearm hour after injury, 2h, 4h, 8h, 12h and 24h experimental group. The lower edge of the experimental group under normal temperature and humidity environment (temperature 22 ℃ -24 ° C, relative humidity of 50% -60%) after anesthesia military 54 pistol shooting injury (shooting point to the rear of the umbilical level 2cm at the right side of the abdominal wall folds hairless District), the establishment of intestinal firearm wound model. Control group, in addition to not injured, and the remaining steps are the same as the experimental group. Each experimental group at the corresponding time points after injury drawn back to the lab immediately drawn to the control group. Groups liver histological changes in the light microscope, simultaneous determination of the level of serum ALT, AST; chromogenic substrate Limulus test detection of plasma endotoxin levels; double antibody sandwich ELISA method for the determination of plasma TNF-α, IL 6 levels; immunohistochemistry image analysis method for the determination of COX-2 expression in the liver tissue. Results: (1) hour after injury of the light microscope, 2h, 4h experimental group to appear gradually increased hepatic cell edema, degeneration, injury 8h, 12h, 24h experimental group appear gradually worsened and spotty necrosis of hepatocytes, focal necrosis, inflammatory cell infiltration. The control group light microscope, no significant changes in injury. (2) the experimental group, serum ALT, AST was significantly higher than that in the control group (P lt; 0.05), two peaks appear after injury 2h and 12h, the 2 peak gt; a peak value ( P lt; 0.05). (3) experimental group plasma endotoxin average significantly higher (P lt; 0.05), peak after injury 8h, 12h after injury remained at peak levels. (4) the experimental group plasma TNF-α, IL-6 were significantly higher (P lt; 0.05), both at 12h after injury peaks. (5) the liver tissue of the experimental group of COX-2 expression was significantly higher (P lt; 0.05), two peaks at 2h after injury and 12h, the 2 peak gt; 1 peak value (P lt; 0.05). (6) The correlation analysis showed that, after injury plasma endotoxin levels and plasma levels of TNF-α, IL-6 levels of COX-2 expression in liver tissue were positively correlated, the correlation coefficients were 0.956,0.927,0.718 (P lt; 0.05 ); injury plasma TNF-α, IL-6 levels of COX-2 expression in liver tissue were positively correlated, the correlation coefficients were 0.854,0.778 (P lt; 0.05). Conclusions: (1) to abdominal firearm injuries result in liver damage of the structure and function of change, along with the extension of time after the injury aggravated. Plasma endotoxin levels were significantly increased (2) after intestinal firearm wound appeared endotoxemia. (3) after intestinal firearm wound endotoxemia can stimulate the body to TNF-α and IL-6 production of liver tissue expression of COX-2, and these inflammatory cytokines mediated liver damage. Complex endotoxin - cytokine network plays an important role in the occurrence and development of secondary liver injury in promoting intestinal firearm wound.

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CLC: > Medicine, health > Surgery > Of surgery > Abdominal surgery > Small intestine
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