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The purpose through animal experiments to investigate the absorption mechanisms and the role of the PLGA membrane prevention after laminectomy epidural scar adhesions. Methods 48 New Zealand white rabbits were randomly divided into A, B, C group, n = 16, A group of gelatin sponge group was the control group, B group and C group PLGA membrane group. The surgical removal of the the L5 lamina caused by of 8mm × 4mm dural bare area, exploration of the nerve root. A group of the dura exposed area no treatment group B coverage gelatin sponge (10 mm × 5mm) the C group coverage PLGA film (10 mm × 5mm). After the first two weeks, four weeks, six weeks, eight weeks, the animals were sacrificed and complete Remove the L5 segments specimens, at each time point. Gross observation, Rydell scar adhesions degree of rating criteria score; After HE staining, microscopic histological observed using a modified Nussbaum standard score and fibroblast cell count; groups epidural scar tissue cut projected electron microscopy. Rydell score, improved the Nussbaum score and the fibroblast line statistical analysis of counting results, take α <0.05 as statistically significant differences in the standard. 48 New Zealand rabbits in the result analysis. ① general observation: group A and group B of epidural scar adhesions gradually increased over time. A group of two weeks epidural adhesions, four weeks, six weeks, eight weeks dural epidural fibrous scar adhesions; B group two weeks began epidural adhesions, four weeks after the gelatin sponge completely absorbed six weeks, eight weeks of extensive fibrous scar adhesions; basic integrity of the C group after 2 and 4 weeks PLGA film, no scar adhesions, six weeks PLGA film begins to degrade, most of the first eight weeks degradation, hard membrane outer surface is smooth and no obvious thickening. No or only a small amount of fibrous scar tissue adhesions. The ② histological observation: four weeks, six weeks, eight weeks of group A and group B epidural surface of a large number of fibroblasts and inflammatory cell infiltration, dural epidural scar tissue adhesions widely. C group at each time point showed no epidural scar tissue, fibroblasts less inflammatory cells compared with A, B two groups had no significant different dura and dorsal tissue adhesions. ③ electron microscopy: A group and B group, a large number of neatly arranged collagen fibers, fiber nucleus, loose chromatin, abundant cytoplasm, visible rich in mitochondria and rough endoplasmic reticulum. Group C, a small amount of disordered arrangement of collagen fibers, even visible fiber cells and fibroblasts scattered cells around collagen fibers. Fiber nucleus concentrated organelles. ④ by SPSS13.0 software line statistical analysis of group C with group A, there were significant differences (P <0.05) and a significant difference (P <0.05), with the presence of group B, group A and group B, no significant difference (P> 0.05) . Conclusion The PLGA membrane and gelatin sponge after laminectomy epidural adhesion prevention research results show that: (1) of PLGA membrane can significantly reduce postoperative epidural fibrous scar formation and subdural adhesions species more satisfactory antiblocking substances; (2) gelatin sponge the epidural adhesion prevention effect is poor, not as an anti-blocking material.
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