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Objective: To detect obstructive sleep apnea hypopnea syndrome (obstructive sleep apnea-hypopnea syndrome, OSAHS) patients with fasting plasma reduced glutathione (reduced glutathione, GSH), oxidized glutathione (oxidized glutathione, GSSG ) and serum reduced coenzyme Ⅱ (reduced coenzyme II, NADPH), oxidized coenzyme Ⅱ (oxidized coenzyme II, NADP) level changes, explore OSAHS and OSAHS patients with hypertension and GSH / GSSG and NADPH / NADP ratio of the change as well as in OSAHS role in the progress of the disease. Methods: random selection of 39 cases of OSAHS patients (all men), were confirmed by polysomnography (polysomnography, PSG) monitoring diagnosis of OSAHS diagnosis is based on the Chinese Society of Respiratory Diseases, sleep apnea disease study group to develop diagnostic criteria, which simple, uncomplicated patients with OSAHS group of 20 patients, aged 29 ~ 60 (42.90 ± 7.60) years of age, body mass index, 22.72 to 34.29 (27.60 ± 2.67) kg/m2. 19 cases of OSAHS patients with hypertension group, age 29 to 69 (48.37 ± 9.44) years of age, body mass index, 22.60 to 33.41 (29.40 ± 3.40) kg / m2, in line with OSAHS diagnostic criteria and in line with the World Health Organization (WHO) in 1979 and International Hypertension League (ISH) (WHO / ISH) developed hypertension clinical diagnostic criteria for hypertension occurred later than OSAHS, and to exclude of secondary nephrogenic endocrine hypertension reasons. 19 cases of normal control group (all men), aged 29 ~ 69 (44.95 ± 10.50) years of age, body mass index, 23.74 to 43.93 (27.26 ± 4.30) kg/m2, upon inquiry, history and line Stardust portable sleep apnea monitor screening examination exclude OSAHS. Comparison between the three groups of age and body mass index without significant differences (all P gt; 0.05), and except for smoking, drinking, diet and drugs interfering factors. All selected objects except for cerebrovascular disease, chronic respiratory disease, ischemic heart disease, secondary hypertension, diabetes, liver and kidney disease, autoimmune diseases. Inclusion in the sleep monitoring end the morning woke Blood samples from 10 ml of 5 minutes, application of colorimetry and enzyme-linked immunosorbent assay detection of plasma GSH and GSSG and serum NADPH, NADP level, and records relating to monitoring indicators, including Sleep apnea hypopnea index (of apnea to hypopnea index, AHI), SaO2 lt; 90% of total sleep time percentage (CT90%), oxygen desaturation index (oxygen desaturation index, ODI), arousal index (arousal index, ArI) . Compared to normal controls, simple OSAHS, OSAHS and hypertension among the three groups plasma GSH and GSSG, the GSH / GSSG and serum of NADPH, NADP, and NADPH / NADP, while relatively simple two groups OSAHS and OSAHS and hypertension, sleep apnea monitoring indicators and the two groups of plasma GSH / GSSG and serum of NADPH / NADP ratio with sleep apnea monitoring indicators linear correlation analysis. Results: 1 plasma GSH, GSSG concentration and GSH / GSSG ratio, serum of NADPH, NADP concentration and NADPH / NADP ratio: normal control group were 246.91 ± 14.76 mg / L, 14.64 ± 5.57mg / L, 21.07 ± 13.71,2.72 ± 0.27 mg / L, and 0.61 ± 0.14 mg / L, and 4.61 ± 1.03, simply OSAHS group were 237.20 ± 16.23 mg / L, 27.96 ± 12.30mg / L, 11.40 ± 7.36,2.17 ± 0.67 mg / L, 1.00 ± 0.14 mg / L, 2.26 ± 0.85, OSAHS complicated with hypertension group were 222.53 ± 14.09mg / L, 40.16 ± 8.03mg / L, 5.81 ± 1.44,1.52 ± 0.38 mg / L, 1.45 ± 0.17 mg / L, 1.07 ± 0.31. OSAHS group alone compared with the normal control group, plasma GSSG and serum the NADP level of elevated, were statistically significant (all P lt; 0.01); plasma GSH, GSH / GSSG and serum NADPH, NADPH / NADP reduce were statistically differences (respectively, P LT; 0.05, P LT; 0.05, P LT; 0.01, P LT; 0.01). OSAHS and hypertension group compared with the normal control group, plasma GSSG and serum the NADP level of elevated, were statistically significant (all P lt; 0.01); plasma GSH, GSH / GSSG and serum NADPH, NADPH / NADP reduce both significant difference (all P lt; 0.01). OSAHS with hypertension group alone OSAHS group, plasma GSSG and serum the NADP level of elevated, were statistically significant (all P lt; 0.01); plasma GSH, GSH / GSSG and serum NADPH, NADPH / NADP reduce both significant difference (all P lt; 0.01). 2 simple OSAHS and OSAHS and hypertension disease two sleep apnea monitoring indicators comparison: with OSAHS merged the hypertension group's AHI, CT90, ODI, ArI rises were statistically significant (t = 2.048,2.110,2.282 2.366; were P lt; 0.05). 3 simple OSAHS and OSAHS and hypertension disease plasma GSH / GSSG and serum NADPH / NADP ratio were positively correlated (r = 0.795, P lt; 0.01; r = 0.600, P lt; 0.01). 4 plasma GSH / GSSG and serum NADPH / NADP ratio with simple OSAHS and OSAHS and hypertension disease group sleep apnea monitoring indicators correlation analysis showed that: ① The plasma GSH / GSSG ratio alone OSAHS group, OSAHS and hypertension group of AHI was negatively correlated (r = -0.853, P lt; 0.01; r = -0.676, P lt; 0.01) and CT90% negatively related (r = -0.687, P lt; 0.01; r = -0.494, P lt; 0.05), and ODI was a negative correlation (r = -0.705, P lt; 0.01; r = -0.467, P lt; 0.05), with ArI a negative correlation (r = -0.495, P lt; 0.05; r = -0.528, P lt; 0.05) ② The serum NADPH / NADP ratio were merged with the simple OSAHS group and OSAHS the hypertension group's AHI was negatively correlated (r = -0.646, P lt; 0.01; r = -0.828, P lt; 0.01), and CT lt; 90% was negatively related (r = -0.799, P lt; 0.01; r = -0.601, P lt; 0.01), the ODI was a negative correlation (r = -0.847, P lt; 0.01; r = -0.839, P lt; 0.01), with ArI showed a negative correlation (r = -0.646, P lt; 0.01; r = -0.665, P lt; 0.01). Conclusion: 1. Except for age, body mass index, and other factors, and the exclusion of smoking, drinking confounding factors such as diet and drugs, as well as the impact of the disease, a simple OSAHS and OSAHS merger of hypertension in patients with plasma GSH / GSSG, serum NADPH / NADP ratio compared with the normal control group decreased, and with AHI, CT90%, ODI, ArI a negative correlation, indicating that both reduced with simple OSAHS and OSAHS patients with hypertension, increased illness and hypoxia. 2. GSH / GSSG and NADPH / NADP body important redox pairs. Numerous studies have shown that the presence of increased oxidative stress in patients with OSAHS, the occurrence of oxidant / antioxidant balance OSAHS development plays an important role. Plasma GSSG and serum the NADP level of in OSAHS patients alone increased, while plasma GSH, GSH / GSSG and serum NADPH, NADPH / NADP reduce OSAHS redox state development direction of oxidized GSSG and NADP level in OSAHS merge high higher blood pressure in patients with GSH, the GSH / GSSG, and NADPH, and NADPH / NADP lower OSAHS patients with hypertension alone OSAHS patients with sleep apnea monitoring indicators were different, that OSAHS patients with hypertension than simply OSAHS patients with more severe disease with OSAHS in the redox state change may be an important factor to promote hypertension occurred. OSAHS is an independent risk factor for hypertension. The degree of change in the redox state of the detection of patients with OSAHS have important theoretical significance and application value for the diagnosis and treatment of patients with OSAHS, condition monitoring and prognostic assessment.
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