Dissertation > Excellent graduate degree dissertation topics show

The Function Role of Cystatin F in Demyelination Disease

Author: LiNing
Tutor: MaJianMei
School: Dalian Medical University
Course: Human Anatomy and Embryology
Keywords: cystatin F demyelination cuprizone cathepsin C microglia
CLC: R744.5
Type: Master's thesis
Year: 2011
Downloads: 12
Quote: 0
Read: Download Dissertation

Abstract


Objective:Study on the effect and its mechanism of cystatin F which is typeⅡcystein proteinase inhibitor in the demyelinating diseases in the CNS.Methods: Cystatin F knockout mice (background of C57B6/129) and littermate wild-type mice (background of C57B6/129) were treated 0.2%cuprizone for creating demyelinating model of central nervous system(CNS). C57B6 mice were blank control. PLP immunohistochemistry(IHC) and Black Gold Staining were used to observe the remaining myelin of cystatin F knockout mice and littermate wild-type mice. In order to clarify the mechanism of demylination which was influenced by cystatin F, IHC and in situ hybridization(ISH) were used to analysis the accumulation of microglia/macrophage lineage cells and the expression of cathepsin C in corpus callosum of cystatin F knockout mice and littermate wild-type mice which were treated cuprizone for 2 weeks or 4 weeks.Results:1.After cuprizone treated for 4 weeks, the area ratio of remaining myelin in cystatin F knockout mice and littermate wild-type mice were both less than that in blank control mice, and the area ratio of remaining myelin in cystatin F knockout mice was significantly less than that in littermate wild-type mice.2. After cuprizone treated for 4 weeks, the number of microglia/macrophage lineage cells and the expression of cathepsin C in the corpus callosum were significantly higher than that in littermate wild-type mice.3. After cuprizone treated for 2 weeks, there was no significant difference of the area ratio of remaining myelin between the cystatin F knockout mice and littermate wild-type mice, even though both of them were less than that in blank control mice.4. After cuprizone treated for 2 weeks, compared with the littermate wild-type mice ,the number of microglia/macrophage lineage cells and the expression of cathepsin C in the corpus callosum of cystatin F knockout mice were not significantly different. Conclusions:1. Lack of cystatin F gene aggravates cuprizone induce demyelination.2. The accumulation of microglia/macrophage lineage cells which was induced by upregulated cathepsin C would be involved in aggravated demyelination in cystatin F knockout mice.3. Lack of cystatin F gene does not elevate the susceptivity of oligodendrocyte to the toxicity of cuprizone.4. The expression of cathepsin C was not effected by cystatin F knockout.

Related Dissertations

  1. The Significance and Level Changes of Urinary Albumin/creatinine Ratio and Serum Cystatin C on Type 2 Diabetic Nephropathy Patients,R587.2
  2. Streptomycin in the Treatment of Trigeminal Neuralgia Research,R745.11
  3. Clinical Study on Cystatin C and Cerebral Ischemic Stroke,R743.3
  4. Correlation Analysis and Clinic Application of Urinary and Serum of Cystatin C,R692
  5. Clinical Significance of Serum Cystatin C and Urinary Cyatatin C in Prinmary Nephrotic Syndrome,R692.3
  6. Cystatin C assessment of chronic kidney disease, renal dysfunction clinical research,R692
  7. Aplication and Development of Cystatin C Kit,R593.241
  8. The Study on the Relationship Between Levels of Serum Cystatin C and Cerebral Infarction and on the Influential Factors of Cystatin C,R743
  9. Estimation of Glomerular Filtration Rates after Orthotopic Renal Transplantation: Evaluation of Cystatin C-Based Equations and Scr-Based Equation,R699.2
  10. A Study on the Correlation Among the Lipid, Cystatin C, High-Sensitivity C-Reactive Protein and the Occurrence, Prognosis of Acute Aortic Dissection,R543.1
  11. The Effect of SSeCKS in Schwann Cells under Inflammation,R363
  12. The Immunity Response in Mice of the Nucleic Acid Vaccine GAPDH in Brugia Malayi and the Gene Cloning of Bm-CPI,R392
  13. The Relationship of Serum Cystatin C and Homocysteine to Diabetic Nephropathy,R692.9
  14. Beneficial Effects of Thymosin β4 and β-aescin on Spinal Cord Injury in the Rat,R651.2
  15. Application of Serum Cys C、Urinary NAG in Clinical Significance of Early Diabetic Nephropathy,R587.1
  16. Relationship between Serum Cystatin C, High Sensitivity C-reactive Protein and Retinopathy in Patients with Type 2 Diabetes,R587.1
  17. The Correlation Studies of Cysteine Protease Inhibitors C and Coronary Heart Disease,R541.4
  18. Construction of Adenovirus Harboured CysC and It’s Effects on the Proliferation of Cultured Rat Cardiac Fibroblast and Collagen Synthesis,R542.2
  19. Clinical Therapeutic Efficacy with Huangqizhimushenqi Granula for Diabetic DKD the Third Stage with Qi and Yin Vacuity,R259
  20. Study on the Correlation between Serum Cystatin C and TCM Syndromes of Diabetic Kidney Disease,R259
  21. Purification and Characterization of CPIs from Silver Carp Egg and Their Application to Prevent Gel Weakening,TS254.1

CLC: > Medicine, health > Neurology and psychiatry > Neurology > Spinal cord disease > Demyelinating disease
© 2012 www.DissertationTopic.Net  Mobile