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Evodia Evodia Rutaceae genus the plant Evodia Evodia rutaecarpa, (Juss.) Benth. The stone tiger Evodia rutaecarpa, (Juss.) Benth.var.officinalis (Dode) Huang or sparse hair Evodia Evodiarutaecarpa (Juss.) Benth.var.bodinieri (Dode ) Huang dried nearly ripe fruit. The Evodia as commonly used in traditional Chinese medicine, mainly for the treatment of abdominal cold pain, vomiting, diarrhea, headache, stomach pain, abdominal pain, dysmenorrhea and high blood pressure and other diseases. Modern studies have shown that it has analgesic, sedative, antibacterial, antihypertensive, anti-hypoxia and other pharmacological effects. Volatile oil (essential oil), a class of plants or herbal aromatic odor, volatile, oily liquid at room temperature, the general term, as the main component of terpenes, sesquiterpenes and their oxygenated derivatives, widely present in the Chinese medicine, spices, often to the level of content to evaluate the pros and cons of the quality of raw material. More systematic study on the the Evodia fruit volatile oil extraction, chemical composition and biological activity. The Evodia volatile oil extraction, separation, purification of the Evodia volatile oil purified, formulated into a solution by gas chromatography analyzer (FID detection device) to optimize the gas chromatographic separation conditions, the inlet pressure 7.32psi, temperature-programmed rate of 8 ° C / min conditions , the separation effect. Comparison of the spectra analyzed by gas chromatography to find that better extraction of petroleum ether. The experimental results obtained major peaks relative retention value of RSD values ??<1.92%; chromatographic peak area RSD <2.36%, indicating good precision instrument, sample measurement has better reproducibility. In addition, compared simultaneous distillation extraction, ultrasonic extraction, soak the extraction method and heat reflux extraction method on the extraction. Concluded: distillation extraction Evodia Extraction of Volatile Oil is better than several other extraction methods. Study to compare and optimize the extraction time, solid-liquid ratio, sample grinding degree of extraction conditions, orthogonal experiment results show that: in the solid-liquid ratio 1:14, extraction time 10h, crushed 20 to 40 mesh (380 to 830μm ), the optimal conditions, volatile oil yield of 0.31%. Evodia volatile oil of the chemical composition using gas chromatography - mass spectrometry (GC-MS) technology combined with Kvoats Retention Index the analysis of the composition of of Evodia volatile oil, separation of from the Evodia volatile oil extracted material in the more than 70 kinds of compounds, and identified out of which 29, the total content of 71%, which is: 3 - carene, alpha-pinene, St. hemp triene, 4 - (1 - methylethyl)-1 - cyclohexen-1 - carboxaldehyde, caryophyllene, thorn core grass-ene, the gamma-Selinene, different speakers ene, 1,4 - two pentylbenzene, aristolochic ene, delta-cadinene Tan purple leukotriene speaker ene oxide II, d - camphene, 15 Methyl alpha-Guaiac wood ene on - Spartak alcohol, cedar enol, cedar enolase, Kauri ene, phthalate, butyl cyclohexyl acetate, isobutyl sub Hong ene, clove eugenol, ocimene, 6,9 - octadecadienoic acid methyl ester, black pine ene. Evodia volatile oil antioxidant activity with K 3 [Fe (CY) system for the determination 6 ] the Evodia volatile oil reducing power; hydroxyl radical system, superoxide anion system the Evodia volatile oil, hydrogen peroxide system and DNA system on the Evodia volatile oil antioxidant activity; In vitro, the concentration of 2.00mg/mL, with steam extraction and reflux extraction radical induced rat liver mitochondrial MDA generated inhibition rates were 65.38% and 61.54%, liver homogenate MDA generated inhibition rates were 21.43% and 31.90% (no induction group), 67.32% and 67.07% (the Fe 2 induction group), 49.54% and 47.43% (H 2 O 2 -induced group); free radical-induced rat liver mitochondria swelling lower than that in the control group. In vivo, the the Evodia volatile oil has to reduce the mice liver tissue lipid peroxidation, showed antioxidant effects in a certain concentration range; also increase serum superoxide dismutase (SOD) activity. Evodia volatile oil vitro antibacterial activity of filter paper method for determination of the Evodia volatile oil antibacterial activity, results showed that in vitro, T (simultaneous distillation and extraction) and H (reflux extraction) sample at a concentration of 10mg/mL, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa inhibition may be related to contained caryophyllene, elemene, eugenol, alpha-pinene and ocimene main component.
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