Dissertation > Excellent graduate degree dissertation topics show

Research Lactarius submerged culture of mycelial

Author: YangPing
Tutor: CaoWenTao
School: Guizhou University
Course: Fermentation Engineering
Keywords: Lactarius hatsudake Tanaka liquid strain submerged cultivation fermentation tank physiological index
CLC: S646
Type: Master's thesis
Year: 2008
Downloads: 160
Quote: 2
Read: Download Dissertation

Abstract


Belongs to the genus of lactarius,Russulaceae family and Russulales,Lactarius hatsudake Tanaka is a precious indigenous mushroom which is mainly distributed in China,Japan etc.It lives in special ecological,nutritional mode and condition to differentiate fruiting,which is symbiosis with Pinus massoniana Lamb,so it can not be cultivated artificially now.So the supply of market is mainly by wild collection, which would lead to serious natural resources destruction because of the intemperance in pluck,and Lactarius hatsudake Tanaka output are gradually decreasing.To protect diversity of edible fungus and meet consumers’ needs,it is an investigatory focus both here and abroad to adopt submerged cultivation techniques for Lactarius hatsudake Tanaka and other domestic funguses.This paper studied and optimized the fermentation medium and the fermentation conditions;and cultivates the mycelium using the rocking bed and the fermentation tank;And the relation between the physiological indexes such as the activity of the chief enzyme,pH value,reducing sugar and the biomass during the progress of the fermentation were studied as well, which provided the scientific bases and technique parameters for the industrial production of Lactarius hatsudake Tanaka.Main findings are as follows:1.Optimized the liquid fermentation medium of Lactarius hatsudake Tanaka on the basis of the single factor test,the result indicates that the maximal mycelia biomass is 0.763 g/100mL under the condition of the liquid fermentation medium of fructose 30.253g/L,peptone 3.576g/L,K2HPO42.5g/L.2.Optimized the liquid fermentation conditions of Lactarius hatsudake Tanaka on the basis of the single factor test.The result is:initial pH 5.0,broth VOL 60mL,the rotary speed of rocking bed 140rpm,injection volume 6%,culture under 27℃were the best and the most mycelia biomass is 0.813 g/100mL which is increased by 6.5 percent than the result pretested using the optimization medium singly.3.The fermentation process of Lactarius hatsudake Tanaka in shaking flask was studied,in which the end of fermentation was determined to be the sixth-seventh day. Amplified the liquid fermentation medium and cultivated the mycelium using the fermentation tank under the optimization fermentation condition on the basis of shake flask test,studied and analyzed the physiological index in the course of the fermentation.

Related Dissertations

  1. The Study of Life Parameters on Different Photopreiods of Mirid and Physiological Index on Host Plant Infested by Mirid,S435.622
  2. Studies of Gene Expression in Puccinellia Tenuiflora under Cd2+ Stress Using Suppression Subtractive Hybridization Technology and Cloning of E2s Gene,S54
  3. In Vitro Induction and Identification of Tetraploid in Malus Zumi and Its Evaluation of Stress-tolerance,S661.1
  4. The Experimental Study on Coupling Effect Mechanism and Model of Crop Response to Water of Uun-sufficient Irrigation under Intercropping Condition,S274.1
  5. Control of Ratio of Higher Alcohol to Ester of Beer in Large Fermentation Tank,TS262.5
  6. Water Quality of Pearl’s West Estuary and Rapid Forecast Determination of Red Tide,X55
  7. The Study of Multivariate Fuzzy Neural Control in the Temperature for Fermentation Process of Beer,TS262.5
  8. Impact of Indoor Air Velocity on Physiological Index and Thermal Comfort,TU831
  9. Studies on the Heat and Freezing Resistance of Hedge Plant in Guiyang,S687
  10. Research on the Relation of Indoor Air Velocity with Human Comfort and Physiological Stress,TU834
  11. On Production, Isolation, Purification and Identification of Alternaria Zinniae Phytotoxin and Development as a Potential Microbial-Product Herbicide,S482.4
  12. Research on Heat Transfer of the Beer Fermentation Tank,TS262.5
  13. Research on the Influence of Indoor Thermal Environment on Body Physiology and Thermal Comfort,TU831.1
  14. Studies on the Methods and Mechanbism of Improving Eggplant Seeds Vigor and Seedling Resistance to Chilling by Seed Priming,S641.1
  15. Isolation and Chracterization of Cold Acclimation Gene (Cpcor413pm1) from Chimonanthus Praecox (L.) Link,S685.99
  16. Study on Knowledge Model-based Expert System for Soybean Management,S126
  17. Effects of Low Temperature Stress on Banana Seedling Physiology Characteristic and Separation of Banana Cold-induced Relative Gene,S668.1
  18. Effects of Different Nutrients and Plant Growth Regulators on the Growth and the Nutrient Uptakes and Utilizations of Winter Wheat,S512.11
  19. Study on Pleurotus Ferulae Liquid Bacterium Seeds Cultivation and Technology,S646
  20. Establishment of in Vitro Propagation Systems and Study on Photoautotrophic Culture of Ilex Chinensis Sims,S687
  21. Studies on the Differences of Physiological Characteristics of Warm Type Wheat and Cold Type Wheat under Different Water-Stress in Seedling Stage,S512.1

CLC: > Agricultural Sciences > Gardening > Vegetable gardening > Fungi ( mushroom )
© 2012 www.DissertationTopic.Net  Mobile