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Endophytic fungi life refers to a period of time in its life cycle within the plant tissue , plant tissue did not cause obvious disease symptoms fungi . Recent studies show that endophytic fungi can produce and host the same or similar metabolites , corresponding endophytic fungi and their metabolites have been looking for a new drug sources via . Valuable medicinal plants - Dendrobium (Dendrobium SW.) For the experimental material from eight kinds of the Dendrobium the root , stem , leaves isolated 92 endophytic fungi , after screening , tassels stem , Su Dendrobium leaves get two charcoal angle bacteria , are numbered LSJ1 and CSY1 induced two bacteria produce sexual reproductive structures ; combination of morphological and molecular biology methods of their identification , identification showed two bacteria are Xylaria venosula ; and determination of the two strains of the end of fermentation , the intracellular and extracellular polysaccharide content of intracellular and extracellular water-soluble protein content , the antibacterial activity . beta - sitosterol tassel the Dendrobium important active ingredients , 12 tassels Dendrobium endophytic fungi PD liquid fermentation , using high-performance liquid chromatography (HPLC) method from one of three bacteria (LSJ1, LSJ5, LSY4) hyphae detected in the β- sitosterol content in the body as follows : 7.94ug / g, 0.274ug / g, 11.51ug / g; morphology and molecular biology method of combining these three bacteria were identified , the identification results show LSJ1 for : Xylaria venosula, LSJ5 belongs Valsaceae, LSY4 : Colletotrichum gloeosporioides . The perennial ( nobile ) Total DNA extraction methods were optimized to improve the extract formulation , improved CTAB concentration (2.5%) and NaCl concentration (1.4mol · L -1 ), and phenol - chloroform extraction of water phase was added 0.5 -fold volume of a high concentration of NaCl (5mol / L) in addition to the polysaccharide , and then add 2 volumes of anhydrous ethanol precipitated DNA receive better results.
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