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A Preliminary Study on Isolation, Identification and Active Ingredient of Endophytic Fungi from Astragalus Membranaceu (Fisch.) Bge. Var. Mongholicus (Bge.) Hsiao

Author: KongXiangJun
Tutor: MaWei
School: Heilongjiang University of Traditional Chinese Medicine
Course: Pharmacognosy
Keywords: Astragalus Endophytic fungi Separation and purification Appraisal Liquid fermentation Active ingredients
CLC: R284
Type: Master's thesis
Year: 2011
Downloads: 64
Quote: 0
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Abstract


Fungal endophytes from medicinal plants in a symbiotic relationship , the endophyte can affect the production and accumulation of the active ingredients of the medicinal plants , Astragalus endophytes of medicinal plants root block has not been reported . Endophytic fungi of Astragalus genus mongholicus separation and identification of endophytic fungi endophytic fungus fermentation conditions optimization and astragalus preliminary study of the active ingredient . First , the choice of four different components of the medium , Mongolia of Astragalus healthy plants tuber materials , surface disinfection method based on the separation of endophytic fungi were isolated from 28 endophytic fungi . The reference fungal identification manual \genus ( 3 ) , bare cell shell case ( 2 ) , branches spike spp , Acremonium genus Penicillium and Aspergillus property of their respective ( a ) . While taking advantage of the five factors endophytic fungi isolated within five liquid fermentation and , in the case of the same formula composition of the fermentation broth , a comprehensive analysis of the fermentation temperature , fermentation time , liquid volume , then the amount of bacteria , shaking speed on fermented dry cell weight , and to explore the interaction between various factors . The results show that slightly different : different endophytic fungi sensitive to the degree of influence factors of different liquid fermentation medium volume , fungal liquid fermentation endogenous mongholicus shaking speed , fermentation time and fermentation temperature , then the amount of bacteria in the present experiment is not the optimal level of the concentrate . Found to meet the medium volume 100ml shaker at 150r/min, temperature 31 ° C , fermentation time of 8 days of fermentation conditions , dry cell weight reached the maximum number of strains up to a comprehensive analysis by the DPS software . In addition , of the 28 endophytic fungi fermentation liquid , the color reaction , TLC detection HPLC detection of the active ingredients of the fermentation broth astrageloside Astragalus flavonoids , APS, three categories were detectable , but not detectable to the three categories ingredients

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