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Fluoroquinolone-resistant Neisseria gonorrhoeae target the enzyme and MtrRCDE efflux system at the same time the study of the occurrence of mutations

Author: FanZuo
Tutor: LiLianQing
School: Shanxi Medical
Course: Immunology
Keywords: Neisseria gonorrhoeae Resistance rates gyrA and parC genes Multi-drug resistant Simultaneous mutations
CLC: R759.2
Type: Master's thesis
Year: 2008
Downloads: 22
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Abstract


Objective: To understand the Shanxi Province, Taiyuan Neisseria gonorrhoeae of five antibiotic resistance rates and resistance characteristics; gonococcal resistance to fluoroquinolones and gyrA and parC gene mutation and mutant distribution; Neisseria gonorrhoeae MtrRCDE discharge system with multi-drug resistant and fluoroquinolone-resistant; fluoroquinolone target enzymes (gyrA and parC genes) and MtrRCDE removal system at the same time the occurrence of mutations. Methods: (1) the disk diffusion method 35 Neisseria gonorrhoeae clinical isolates to penicillin, tetracycline, ciprofloxacin, ceftriaxone, the Grand View enzyme five drugs susceptibility testing. E test measures 35 gonococcal MICs of ciprofloxacin. (2) was amplified by PCR gyrA the parC gene and mtrR of promoter region 13bp palindromic sequence. (3) the gene sequencing of target gene sequence analysis. Results: (1) in Taiyuan area Neisseria gonorrhoeae to penicillin, tetracycline, ciprofloxacin, ceftriaxone, spectinomycin enzyme resistance rates were 68.6%, 74.3%, 100%, 11.4%, 2.9%, while resistance to the two kinds of above multiple antimicrobial resistance rate of 80.0%. (2) resistance to fluoroquinolones in Neisseria gonorrhoeae 35 there are the gyrA gene single-or double-digit point mutations, mutations mainly in the form of Ser-91 → Phe and Asp-95 → Gly. 25 plant height level of fluoroquinolone-resistant bacteria (MIC ≥ 4mg / L) there are double-digit points in gyrA gene and parC gene single or multi-point mutation. parC gene mutation most common type is the double-digit point mutation of Ser-87 → Arg Tyr-104 → Tyr. (3) of 27 in the promoter region of the 28 multi-resistant strains a mutation, and the type of mutation in the palindromic sequence of 13 nucleotides, a single A / T base deletion. (4) gyrA, parC and mtrR promoter District mutation frequencies were 100%, 94.3%, 77.1%. 77.1% of the strains gyrA and promoter region mtrR, while mutation. Conclusions: (1) in Taiyuan, Shanxi gonorrhoeae growth rate of ciprofloxacin resistance and multi-drug resistant rate rapidly the the Grand View enzyme and ceftriaxone is still available as the drug of choice for treatment of gonorrhea in the region. (2) parC gene mutations caused by Neisseria gonorrhoeae is highly resistant to quinolones parC gene mutation is more, the higher the level of the MIC. (3) the mtrR promoter region the palindromic sequence a single A / T base deletion and Neisseria gonorrhoeae high level of multi-drug resistance are closely related. (4) the fluoroquinolone target enzyme and MtrRCDE of discharge system mutation is an important mechanism for Neisseria gonorrhoeae to fluoroquinolone resistance.

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CLC: > Medicine, health > Dermatology and Venereology > Venereology > Gonorrhea
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