|
Objective: the concept 察厄贝沙坦 (Irbesartan) of streptozotocin (STZ) diabetic rat kidney cortex glucose into protein 1 (GLUT-1) and transforming growth factor β1 (TGFβ1) expression, and to explore the possible mechanism. : Wistar rats 48, were randomly divided into normal control group (14), diabetes (17) and irbesartan treatment group (17). (2) The diabetic group and irbesartan treated rats given streptozotocin (STZ) 55mg/kg intraperitoneal injection of a diabetic rat model, normal group was given volume of citrate buffer intraperitoneal injection. Model after 8 and 12 weeks in each group were sacrificed four rats and collecting specimens, recording weight, testing blood sugar, serum creatinine, blood urea nitrogen, blood cholesterol, blood triglycerides, 24h urinary protein excretion. By RT-PCR method to detect the renal cortex the GLU-1mRNA and TGFβ1mRNA expression levels. Renal tissue hematoxylin - eosin (HE), periodic acid - Schiff reagent (PAS) staining. Results: (1) diabetic rats, blood glucose, serum creatinine, blood urea nitrogen, blood cholesterol, blood triglycerides, 24h urinary protein excretion was significantly higher than the normal control group (P <0.01). The kidney tissue GLUT-1mRNA expression and TGT-βmRNA, expression are significantly higher than the normal control group (P <0.01). Kidney of diabetic rats with HE and PAS staining showed pathological changes characteristic of diabetic nephropathy - glomerular hypertrophy, mesangial area expansion, vacuolar degeneration of tubular epithelial cells, suggesting that the model in diabetic nephropathy stage. (2) irbesartan treatment group blood cholesterol downward trend, with the time of treatment in the 12th week and four weeks, the difference was highly statistically significant (P <0.01). Irbesartan treatment group, serum creatinine, blood urea nitrogen, and 24 h urinary protein excretion in 4 weeks diabetic group differences are highly statistically significant (p <0.01) to 12 weeks the difference is particularly significant; irbesartan treatment group kidney tissue GLUT-1 and TGT-β1 mRNA expression was significantly lower than the diabetic group (P <0.01). Kidney HE, PAS staining kidney pathology changes reduced. Conclusion: irbesartan-treated rats urine protein excretion decreased significantly compared to the diabetic group, blood urea nitrogen, creatinine decreased, while renal pathological changes reduce and kidney tissue GLUT-1 and TGT-β1 mRNA expression was significantly reduced, suggesting that effectively prevent or delay the progress of DN pathology, a protective effect of DN.
|