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Objective: To observe the end of the rat vascular calcification model cardiovascular tissues vasopressin II (urotensin II, UII) and its receptor (urotensin II receptor, UT, or GPR14) expression changes, explore UII and its receptor in vascular calcification in significance. Method: 9-week-old male SD rats were randomly divided into four groups of 10 each, respectively, for the normal control group, the calcified group (on the first day of vitamin D3 intramuscular injection of 300,000 U / kg, while nicotine, 25mg/kg, irrigation stomach after conventional breeding), calcified group of L-arginine (1g/kg, orally four weeks, daily) and calcification methionine (1g/kg, orally for 4 weeks, once daily) 4 weeks after the experiment, rats were measured vascular calcium content, alkaline phosphatase (ALP) activity and Von Konsa staining to determine the degree of vascular calcification; UII protein content measured by radioimmunoassay in rat plasma, aortic and myocardial UII protein; immunohistochemistry assay cardiovascular tissue; RT-PCR assay cardiovascular tissue the UT mRNA level. The experimental results using Prism 4 statistical software analysis. Results: Von Kossa staining to the film black particles and lumps in the calcification of blood vessels (aorta) precipitation, the most obvious to the calcified group and calcification methionine. Compared with the normal control group, vascular calcification and calcium content in the calcified group, calcification of the L-arginine group and calcification methionine group increased by 2.2-fold (P lt; 0.01) and 1.8-fold (P lt; 0.05) and 6.9 times ( P lt; 0.01); ALP activity in the calcified group increased by 1.7-fold (P lt; 0.05), the calcified group of L-arginine and calcification methionine group and normal group but no significant difference; vascular UII content in the calcified group the calcification L-arginine,, with calcification methionine group increased by 3.0-fold, 2.6-fold and 2.9-fold (P lt; 0.05), immunohistochemistry results showed that the calcified group, calcification of the L-arginine group and calcification methionine group UII express high in the control group, the results of radioimmunoassay results are consistent, but no significant difference in the plasma UII content; calcified tissue UT mRNA upregulation in vascular calcification group, calcification of L-arginine and calcification methionine group increased 1.8 times (P lt; 0.05), 1.5-fold (P gt; 0.05) and 2.7-fold (P lt; 0.01), and myocardial mRNA were increased 1.9-fold (P lt; 0.05) and 1.3-fold (P gt; 0.05) and 2.2 times (P lt; 0.05). Conclusion: UII and its receptor in the rat cardiovascular calcified tissue expression was significantly increased, suggesting that UII might be involved in the development of vascular calcification process, UII may be a new factor promote vascular calcification.
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