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In recent years, the special physical properties of nanomaterials, aroused people's attention, scientists began to apply it to the prevention, diagnosis and treatment of the disease in the biomedical field, especially medicine tissue reconstruction. Due to its own tissues and organs nanoscale, nanomaterials, biomimetic characteristics and the physical properties of the conducting tissue reconstruction stimulate cell growth, guided tissue repair. Although this is a new field, but many scientists have designed and synthesized a the biomimetic material encapsulated cells (such as stem cells, cartilage cells, bone cells, etc.) used in tissue engineering. However, the ultra-small particle size of the nano-materials to make it through a variety of ways into the body, such as bone marrow. When the nanoparticles into the bone marrow, the balance of bone metabolism and bone tissue will be affected and we need to carry out in-depth research. Therefore, bone marrow stromal cells and osteoblasts as a model, tetrazolium (MTT), alkaline phosphatase activity and oil red O staining and quantitative measurement methods at the cellular level of the gold nanoparticles (Au NPs) and carbon nanotubes (CNTs) on the proliferation of murine bone marrow stromal cells, osteogenic, adipogenic differentiation into bone cell proliferation and horizontal differentiation by immunoblotting (Western blot) and real-time quantitative polymerase chain reaction ( Q-PCR) methods such as osteogenic and adipogenic differentiation of bone marrow stromal cells process related proteins and genes have been studied. The results show that: 1. Cellular level gold nanoparticles can promote murine bone marrow stromal cells, primary bone cells osteogenic differentiation, inhibition to the adipogenic and horizontal adipogenic differentiation; cellular level carbon nanotubes can be inhibition of mouse bone marrow stromal cells, primary bone cells into bone into fat and horizontal adipogenic differentiation; 3 gold nanoparticles increases osteoblast differentiation (Runx2, BMP-2, OCN, ALP) gene expression, down adipogenic differentiation-related genes (PPARγ2, E / CBPα, E / CBPβ and of E / CBPδ) expression; 4. carbon nanotubes down into the bone into adipogenic differentiation related gene expression; 5 gold nanoparticles increase in mouse bone marrow stromal cells, the original Runx2 and BMP-2 protein expression in the process of generation of bone cells into osteoblasts. The above results, the gold nanoparticles promote osteogenic differentiation and inhibition of adipogenic and adipogenic transdifferentiation, carbon nanotubes inhibited bone marrow stromal cells into the bone into a fat differentiation and primary osteoblasts osteogenesis and transverse Grease differentiation.
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