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The Study on the Extraction Method of Biotransformation and Cosmetic Efficacy of Oat β-glucan

Author: PanZuo
Tutor: HeCongFen；WangChangTao
School: Beijing Technology and Business University
Course: Biochemical Engineering
Keywords: Biotransformation β-glucan Cosmetic effects
CLC: TQ652
Type: Master's thesis
Year: 2010
Downloads: 112
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Abstract


Oat β-glucan is a sub-aleurone layer of the oat grain cell wall of a non-starch polysaccharides. Oat β-glucan for its nutritional value, the value of the medical care and feeding value and attract attention. Oat β-glucan by grinding concentrated in the bran of oat bran as the research object, research oat β-glucan extracted by biotransformation process, separation and purification, physical and chemical properties as well as its cosmetic effects. Biotransformation divided into enzymatic conversion and microbial transformation. By orthogonal experiment to optimize the traditional water extraction and enzymatic conversion method, to compare extraction process and oat β-glucan yield results showed that the enzymatic conversion of the extraction rate is higher than the traditional water extraction. By orthogonal experiment to optimize the the the optimal enzyme mentioning conditions for liquid ratio of 15:1, enzymatic hydrolysis time of 3 h of 3%, plus the amount of enzyme. Enzyme mention orthogonal best combination of β-glucan rate 19.57mg / g. The microorganisms directly access the oat bran and water mixture in the fermentation, a low yield of β-glucan. Improved technology, oat bran before yeast fermentation with the No. 5 and No. 6, β-glucan increased yield. Oat bran medium should be in the yeast pre-fermentation processing with proteases optimum conditions, the yields were 22.63mg / g and 22.72mg / g. Oat β-glucan containing starch, protein, sugar, and other miscellaneous impurities in the crude extract. The high temperature α-amylase to remove starch, the isoelectric point of the protein precipitation, alcohol precipitation of the β-glucan was purified. By GC analysis of the monosaccharide composition of purified β-glucan to glucose. GPC analysis of the average molecular weight, water extraction is 9.697 × 10 ~ 5, the enzyme mention converted to 1.634 × 10 5, fermentation of 1.322 × 10 5, whose radius is 28.6nm. Franz diffusion cell analysis transmittance, and the results showed that β-glucan through lower fermentation gt; enzymatic gt; water extraction. Through the anti-aging effect into the fiber cell cultures and skin texture degree human experimental study β-glucan. The results show that: the concentration of the β-glucan of 0.005% all can fibroblast proliferation; smear add cosmetic four weeks after the β-glucan, the skin texture diagram shows the β-glucan can lighten skin texture. Β-glucan determination of the MMV and TWEL value study moisturizing effect. The results show that: the cosmetics can be applied to add the β-glucan to increase skin moisture and prevent moisture loss. Experiments show that water extraction, enzymatic conversion method and fermentation of oat β-glucan extract added to cosmetics moisturizing and anti-aging effect. According to the above experiments, we can know biotransformation Extraction oat β-glucan, and its yield is improved than the traditional water extraction, and the molecular weight is less than the traditional water extraction through skin amount slightly higher than the water extraction. Biotransformation extraction of oat β-glucan and water extraction oat β-glucan moisturizing and anti-aging effect is similar.

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