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This article was prepared by dispersion polymerization micron monodisperse poly glycidyl methacrylate (Poly (Glycidyl Methacrylate), PGMA) microspheres. Experiment azobisisobutyronitrile (2,2 '-azo-bis-isobutyronitrile, (AIBN)) as initiator, polyvinylpyrrolidone (polyvinylpyrrolidone, (PVP)) as dispersing agent, ethanol and water as reaction medium, of the initial monomer (GMA) concentration, PVP concentration and reaction temperature on PGMA microsphere size and dispersion effects, the results are displayed in the initial monomer concentration of 15 wt%, PVPK-30 concentration of 15wt%, reaction temperature 70 ℃, polymerization under the conditions of time more than 12 hours to obtain a uniform microsphere size, morphology rules successfully prepared in this condition, the particle size in the range 1.7-3.1μm, variation coefficient of less than 5% of PGMA monodisperse microspheres. PGMA microspheres using ethylenediamine on the surface functional modification, FTIR analysis showed that the surface of the microspheres in PGMA successful introduction of an amino group, double jump titrating amino group content, the results show microspheres amino content of about 3mmol / g. Amination to produce monodisperse PGMA microspheres as a carrier, the use of in situ synthesis and swelling are two ways to penetrate successfully prepared both fluorescent and magnetic properties of multifunctional polymer microspheres, the microspheres morphology multifunctional scanning electron microscope and calculate the mean diameter and coefficient of variation, showing that the microsphere surface is smooth, uniform particle size distribution, was monodisperse; XRD and VSM analysis shows that success in microspheres in situ generated magnetic nanoparticles, superparamagnetic microspheres the saturation magnetization of 4.6emu / g; fluorescence microscope to the multifunction microspheres has strong fluorescence intensity; ultraviolet light separation experiments lower magnetic microspheres can be visually observed in the absence of external magnetic field, can be a stable suspension in water, under the action of an external magnetic field, can quickly gather separation, indicating that both superparamagnetic microspheres and fluorescence properties. Prepared by the swelling amination penetration of magnetic microspheres used in multi-fluorescence immunoassay, using glutaraldehyde activated microspheres multifunctional amino, connection goat anti-rabbit IgG antibody, with the connection with goat anti-rabbit IgG Multi Microspheres rabbit IgG detection function and chicken IgG, magnetic separation to remove unreacted microspheres showed multifunctional connection microspheres antibodies can be easily and can maintain the biological activity of the antibody, specific adsorption of the antigen corresponding to the antibody, and having a small non-specific adsorption, the fluorescent microscope can clearly distinguish the negative and positive controls. Multi microspheres while flow cytometry, and the results show multifunction size microspheres have a uniform, a uniform fluorescence intensity, can easily be detected by flow cytometry signals can be applied by flow cytometry automated immunoassay.
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