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The Application of Rare Earth Oxide in the Direct Electrochemistry of Redox Proteins

Author: LiYan
Tutor: LiuJinRong；GaoYanFang
School: Inner Mongolia University of Technology
Course: Chemical processes
Keywords: Ceria Thulium oxide Hemoglobin Glucose oxidase Direct electrochemical
CLC: O629.73
Type: Master's thesis
Year: 2010
Downloads: 59
Quote: 0
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Abstract


Redox proteins and enzymes are a class of biological macromolecules and a special catalyst, they are involved in the completion of the system of life in many physiological processes, these processes go through the process of electron transfer, redox proteins and enzymes direct electrical The chemical helps to obtain the thermodynamic and kinetic properties of the protein, which laid a foundation for revealing biological redox process, a very important theory and application of guiding significance for the development of novel biosensors, fuel cells and other biological electronic devices. The This article select rare earth oxides CeO 2 Tm 2 O 3 the model molecule hemoglobin (Hb) as redox proteins and enzymes and glucose oxidase The carrier (GOD), to prepare a series of enzyme electrodes, and investigated electrochemical method they are in Hb and GOD direct electron transfer bioelectrocatalysis of the application. The results show that: (1) the average particle diameter of 179.1 nm, CeO 2 Material obtained by carbonate precipitated decarburization method. Its application to the production of the enzyme electrode, making fixed in CeO 2 Hb and GOD on material not only effective electrode surface for direct electron transfer, and are able to maintain their biocatalytic activity. Prepared Nafion / the CeO 2 / Hb / GC modified electrode electron transfer rate ks (0.68 ± 0.09) s -1 H 2 O 2 detection limit for 1.013μmol the · L -1 , good reproducibility and stability; obtained GOD / CeO 2 / Nafion / GC modified electrode electron transfer rate ks (3.89 ± 0.19) s -1 , the linear range for glucose detection 0.5 4 .5 mmol · L -1 , good reproducibility and stability. (2) the average particle size of 10 nm Tm 2 O 3 materials prepared by hydrothermal synthesis and its application to the production of the enzyme electrode. The results showed that the fixed Tm 2 O 3 Hb on the material and GOD, not only to the electrode surface effectively direct electron transfer, but also be able to maintain its biocatalytic activity. Prepared Nafion / Tm 2 O 3 / Hb / GC modified electrode electron transfer rate ks was 0.98 s -1 of H < sub> 2 O 2 detection limit for 1.813μmol · L -1 , good reproducibility and stability; obtained Nafion / Tm of the 2 O 3 / GOD / GC modified electrode electron transfer rate ks is (3.27 ± 0.43) s -1 glucose detection limit can reach 7 mmol · L -1 , good reproducibility and stability.

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CLC: > Mathematical sciences and chemical > Chemistry > Organic Chemistry > Natural compounds > α-amino acids,peptides, proteins, nucleic acids > Protein
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