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Nanostructured Materials Enhanced the Direct Electron Transfer of the Immobilized Protein and Its Application for Biosensing

Author: ZhangZuoZuo
Tutor: YangXiaoDi
School: Nanjing Normal University
Course: Physical and chemical
Keywords: Multi-walled carbon nanotubes Nanocrystalline Zirconia Hemoglobin Glucose oxidase Cytochrome P450 2B6 Polyurethane
CLC: O629.73
Type: Master's thesis
Year: 2007
Downloads: 133
Quote: 0
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Abstract


Direct electron transfer has important theoretical and practical value in the study of protein between the electrodes. The protein is in close contact with the electrode surface will cause important changes in the structure and function of the protein, modified electrode or protein, direct electron transfer properties of the protein can be improved. Carbon nanotubes and zirconium dioxide nanoparticles to provide a micro-environment that is similar to the its natural system is to be able to be fixed on the protein in its molecular orientation to the protein molecules more freedom shell direct electron transfer protein molecule, thus reducing the insulating properties , and through the channel of nanoparticles accelerate the process of electron transfer. The papers protein molecules on the nanoparticle fixed, electron transport and sensing applications to carry out the following tasks: (1) carbon nanotubes enhanced electron transfer protein in low conductivity matrix and its sensing study of hemoglobin (Hb fixed) in the polyurethane film (PUE) and its direct electrochemical properties. Polyurethane is a film-forming properties and good stability of the polymer materials, but its conductivity and the permeability is poor. Infrared spectroscopy studies have shown that the the Hb molecule fixed to the PUE film maintained its natural active nanotubes added to the above system, IR spectroscopy showed the still characteristic peaks of the Hb molecule appears. Hb / PUE modified graphite electrodes in 0.1 mol / L, pH 7.0 phosphate buffer solution (PBS) cyclic scan obtained one pair of redox peaks, but the peak current is small, Hb / PUE / MWNTs modified graphite electrode in the system circulates scan cyclic voltammetry stable and better peak shape redox peaks, peak current increased 4 times, the formal potential E. 0 = -334 mV (vs Ag / AgCl). With the increase in the pH of the solution, the formula weight redox potential and the pH value in a straight line relationship, the slope of -54.47 mV / pH (R = 0.993). The comparison shows that the peak current nano TiO 2 and ZrO 2 added increased by 1.5 and 2.5 times than not Jia Nami particles. Scanning electron microscope (SEM) The results show that the carbon nanotubes are added Hb / PUE film is a surface cleaning, uniform particle size, the porous mesh structure, so that Hb / MWNTs / PUE / PG modified electrode showed a stable and sensitive electrochemical response. Using this modified electrode to detect NO 2 - , can be more sensitive electrochemical response, a linear range of 0.08-3.6 mmol / L, the detection limit was 6.8μmol / L. (2), glucose oxidase fixed biosensing research on zirconium dioxide nanoparticles fixed in zirconia nanoparticles (ZrO 2 ) modified cleavage glucose oxidase on a graphite electrode (PG) (GOD) direct electrochemical behavior and electrocatalytic properties. Were used with positive or with negative electric platinum gum solution (Pt-PLL, and Pt-PVA), and the DMSO solution to Preparation of ZrO the uncharged 2 nanoparticles modified electrode was found that nanoparticles charging properties of the enzyme direct electron transfer properties have a significant impact. The formal potential of various electrodes, the amount of adsorbed protein, and the electron transfer rate. The experiments show that the ZrO 2 nano-particles, gold glue and PVA hybrid modified GOD electrode showed the fastest rate of electron transfer and minimal formal potential E 0 . ZrO 2 / Pt-PLL and of ZrO 2 / Pt-PVA-nano-particles on the glucose oxidase enzyme molecules can maintain its biological activity and natural structure, fixed in glucose better electrocatalytic response in did not detect the ZrO 2 / DMSO and ZrO 2 / Didodecyldimethylammonium Bromide modified electrode to GOD activity. (3) The cytochrome P450 2B6 zirconia nanoparticles modified electrode on the electrochemical behavior of zirconium dioxide nano-particles and mixed Pt-PLL modified electrode immobilized cytochrome P450 has a relatively good electrochemical signal, making direct electron transfer rate between the P450 and the electrode increases. The nano of ZrO 2 particles and the Pt-PLL have played a role in enhanced electron transfer immobilized the CYP2B6 to oxygen exhibit excellent electrocatalytic signal, by anticancer drug lidocaine can be added to the buffer solution promote electrocatalytic oxygen reduction reaction, to adopt the amperometric can build a P450 substrate drugs detection sensor.

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CLC: > Mathematical sciences and chemical > Chemistry > Organic Chemistry > Natural compounds > α-amino acids,peptides, proteins, nucleic acids > Protein
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