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Biomimetic hydroxyapatite / chitosan - gelatin network Composites

Author: LiJunJie
Tutor: YaoKangDe
School: Tianjin University
Course: Materials Science
Keywords: Nanohydroxyapatite Chitosan Gelatin In situ deposition Cancer cell Bone marrow stromal stem cells Bone Tissue Engineering
CLC: R318.08
Type: Master's thesis
Year: 2007
Downloads: 211
Quote: 0
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Abstract


Synthetic bone substitute materials is essential for the treatment of bone defects, the U.S. Food and Drug Administration (FDA) has not approved combination with cells or growth factors for bone tissue engineering devices. Natural bone is composed of hydroxyapatite and collagen fibers of the organic / inorganic composite, therefore, the formation of nano-hydroxyapatite / polymer composite materials as well as for the provision of bone tissue biocompatibility overall combined with a very important role. This article from the perspective of bionic, chitosan - gelatin network film (CG) soaked in Ca (NO 3 ) 2 -Na 3 PO 4 of Tris buffer solution in situ formed by deposition on the surface of hydroxyapatite (nHA), by reflection infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD) and thermal gravimetric analysis Crystal Research and CG nHA interaction between the membrane, while the projection by XRD and electron microscopy study of the influence factors nHA crystal particle size, such as complexes of chitosan - gelatin ratio of calcium ion concentration and the reaction temperature. The results showed that, carboxyl, carbonyl and amino groups in the formation of the HA plays a very important role, nHA crystals with an average particle size of gelatin content increases and decreases calcium concentration is reduced, also found at the temperature below 50 ℃ particle size had no effect (17.2-19.2 nm between), but when the temperature is increased to 70 ℃, the particle size increased rapidly to 52.3nm. Cytocompatibility biological material must have the performance, one article in the preparation nHCG composites based on the study of gastric cancer cells and bone marrow stromal cells (MSCs) in nHCG micron composite membrane and hydroxyapatite / chitosan - gelatin network composite membrane (mHCG) on cell behavior, experiments show that, nHCG composite composites more than mHCG inhibit cancer cell growth and with the composite material nHA content increases the stronger the inhibition; hand, nHCG able MSC promote cell adhesion and growth with induction of differentiation can promote their exhibit high alkaline phosphatase activity. Were prepared using the phase separation CG network rack, and the surface of the stent in situ deposition nHA crystals formed nHCG bracket. Found nHCG scaffold has a high porosity and a suitable pore size, but also has a strong mechanical properties. In SBF, nHCG stand its environment and the pH value was less affected, the degree of swelling is also smaller. On this basis, construct a miniature pig osteoblasts / nHCG porous scaffold structures, materials prepared from the perspective of science and engineering organizations opened their basic research.

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