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Resveratrol is a plant antibiotics. It is found mainly in the grape skin, peanuts, mulberry, pine (for example, in Scotland the pines, East Fangbai Song, etc.), Polygonaceae plants (giant knotweed, Chinese medicine Polygonum cuspidatum) 12 families, 31 genera of 72 kinds of plants. The first purified resveratrol in 1974, the plant source is a growth in Peru legumes. Resveratrol is organisms resistant to environmental stress (such as bad weather, microbial infection, etc.) and chemical substances. Pharmacological and biological effects of resveratrol has significant anti-cancer, protect nerves, anti-aging, anti-inflammatory, and prolonging life. \The study found that this is because the French table wine contains resveratrol. In this work, Polygonum cuspidatum raw materials, compared dipping method, a Soxhlet extraction method, and the ordinary reflow method, to determine the process conditions of a crude extract of resveratrol: 4 times, 3 times, respectively, 3 times the volume of an aqueous solution of 80% ethanol three times to boiling Polygonum cuspidatum powder extract resveratrol, then the combined three extracts were concentrated to a paste. The paste was diluted with 200ml of water, heated to 60 ℃, filtered hot. The four extracted with 300ml of ethyl acetate and after the filtrate was cooled, the combined ethyl acetate phase was concentrated to dryness. Through the application of macroporous resin - the silicone joint chromatography method, purified resveratrol from Polygonum cuspidatum extract. Improved macroporous resin, silica gel or ion exchange resin single column chromatography purification of resveratrol from Polygonum cuspidatum traditional crafts. In the the resveratrol extraction costs are not greatly improve the extraction purity. Macroporous resin - Silicone Joint chromatography resveratrol have not been reported in the literature. The eluent used in the text component unchanged, to overcome the result of the gradient elution should not be repeated, and elution conditions are complex defects. Then analyzed and studied the chromatographic conditions, determine the first macroporous resin chromatography, eluent ethyl acetate - ethanol mixed solution, the volume ratio of 3:2, the elution rate 1mL/min; then purified by silica gel chromatography eluent ethanol - ethyl acetate mixed solution, the volume ratio of 4:1, elution rate 1min/ml. Resveratrol purity above two column step-by-step chromatography by HPLC analysis of the resveratrol content of about 87%. MS analysis of the composition and purity of the product, the results obtained with the HPLC analysis results anastomosis.
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