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Objective To detect platelet reagin -1 (TSP-1), vascular endothelial growth factor (VEGF) and microvessel density (MVD) expression in chronic cervicitis, the like lesions cervical intraepithelial neoplasia (CIN) and cervical cancer tissue role in the development and angiogenesis correlation of TSP-1 and VEGF in cervical lesions occurred. Methods Immunohistochemical SP method detected 10 cases of chronic cervicitis, 13 cases of CIN Ⅰ, 22 cases of CIN Ⅱ ~ Ⅲ and 48 cases of cervical cancer tissue TSP-1 expression of VEGF and CD34 antigen as an endothelial cell marker measured MVD. Results 1. TSP-1 in chronic cervicitis, CIN I, CIN of Ⅱ ~~ Ⅲ and cervical cancer tissue positive expression rate of 90.00%, 76.92%, 36.36% and 29.16%, respectively. TSP-1 is mainly expressed in the basal cell layer chronic cervicitis and CIN Ⅰ organization, TSP-1 expression gradually decreased with increasing cervical lesions level. TSP-1 expression was significantly lower than in the group of cervical cancer and CIN Ⅱ ~ Ⅲ chronic cervicitis and CIN Ⅰ group, the difference was significant (P lt; 0.05). Chronic cervicitis and CIN Ⅰ, TSP-1 expression differences between CIN of Ⅱ ~~ Ⅲ and cervical cancer group had no statistical significance (P gt; 0.05). TSP-1 expression and cervical cancer histological grade and lymph node metastasis related (P lt; 0.05). The positive expression rate of VEGF in chronic cervicitis, CIN Ⅰ, CIN of Ⅱ ~~ Ⅲ and cervical cancer tissue were 0,23.08%, 40.90% and 66.67%, respectively. Chronic cervicitis tissues not found the expression of VEGF, VEGF expression gradually increased with increasing cervical lesions level of VEGF expression in cervical cancer tissue was significantly higher than that of chronic cervicitis, CIN Ⅰ and CIN Ⅱ ~~ Ⅲ group, the difference was significant (P lt; 0.05), CIN Ⅱ to Ⅲ group VEGF expression than chronic cervicitis group, the difference was significant (P lt; 0.05). VEGF expression between CIN Ⅰ and chronic cervicitis and CIN Ⅱ ~~ Ⅲ group there was no statistically significant difference (P gt; 0.05). VEGF expression in cervical cancer clinical stage, histological grade and lymph node metastasis (P lt; 0.05). 3. MVD chronic cervicitis, CIN Ⅰ, CIN Ⅱ ~ Ⅲ and cervical cancer tissue were 6.8 ± 1.9,13.0 ± 3.6,22.7 ± 4.4 and 31.0 ± 5.3. MVD gradually increased with increasing cervical lesions level differences between groups were significant (P lt; 0.05). MVD with cervical cancer histological grade and lymph node metastasis (P lt; 0.05). Cervical lesions TSP-1 and VEGF expression intensity was a significant negative correlation (r = -0.358, P lt; 0.05), TSP-1 expression intensity and MVD was negatively correlated (r = -0.624, P lt ; 0.01), and VEGF expression intensity and MVD was positively correlated (r = 0.538, P lt; 0.01). Cervical cancer, VEGF expression group, MVD was significantly higher than that VEGF expression group, the difference was significant (P lt; 0.05). TSP-1 expression group, MVD was significantly lower than the TSP-1 expression group, the difference was significant (P lt; 0.05). The expression of VEGF and the MVD count higher than the TSP-1 expression in cervical cancer tissue VEGF expression of TSP-1 expression, the difference was statistically significant (P lt; 0.05). VEGF and TSP-1 expression in cervical cancer tissue positive significant difference (P lt; 0.05). Conclusion TSP-1 and VEGF expression of both unbalanced early precancerous lesions of the cervix appears high and low expression of TSP-1 and VEGF expression may be involved in the process of development and angiogenesis cervical lesions occurred, decided to cervical cancer angiogenesis and tumor development, one of the key factors. TSP-1, VEGF expression and MVD determination can be judged by the indicators of the degree of development of cervical lesions. Combined detection of TSP-1, VEGF and MVD conducive to early diagnosis and early treatment of cervical lesions.
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