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The Analysis of Current Situation of Bacterium Resistance to Antibiotic in Suzhou and the Detection of Partly Antibiotic-resistance Bacterium and Their Genotype Distribution

Author: HaoWeiMin
Tutor: ShenJiLong；XuYuanHong
School: Anhui Medical University,
Course: Pathogen Biology
Keywords: Antimicrobial resistance monitoring Microbial sensitivity tests Resistance Reference Standards Extended-spectrum β -lactamase Antimicrobial drugs
CLC: R450
Type: Master's thesis
Year: 2008
Downloads: 49
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Abstract

Objective To investigate the clinical isolates of common bacteria and drug resistance, and resistance to be analyzed for clinical rational choice of antibiotics to provide an accurate basis. Methods All bacteria identified by conventional methods; drug susceptibility testing using agar disk diffusion method (ie KB method), and press the 2005 American Society for Clinical Laboratory Standards Board standard guidelines for calculating bacterial antimicrobial resistance rate and sensitivity rate. Results are collected in Suzhou City Hospital, the first three of the 631 isolates of bacterial resistance monitoring data. Common strains of Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus and coagulase-negative staphylococci; isolates conducted according to different types of bacteria strains (genus) resistance rate analysis, found that 2005 annual extended-spectrum β-lactamase of Escherichia coli and Klebsiella pneumoniae strains isolated were 64.7% and 60.5%, MRSA (methicillin-resistant Staphylococcus aureus) The separation rate of not less than 46.2%, MRCNS (methicillin-resistant coagulase-negative staphylococci) separation rate of 48.3%, the majority of Gram-negative bacilli to imipenem, meropenem, piperacillin / tazobactam, cefoperazone / sulbactam; did not find the glycopeptide antibiotic-resistant Staphylococcus aureus. Conclusion Because of the city common strains commonly used in clinical drug resistance rates continue to increase, should strengthen the monitoring of drug resistance of pathogens. Objective To study the Suzhou City in clinical isolates of Escherichia coli and Klebsiella pneumoniae ESBLs detection rate of drug resistance and genotype distribution. Methods 2005 CLSI recommended method for collecting ESBLs strains confirmatory test; agar plate dilution method for determination of ESBLs producing Escherichia coli and Klebsiella pneumoniae to 13 kinds of antimicrobial drug resistance; then produced by PCR method ESBLs strains using eight pairs of primers for β-lactamase gene amplification, PCR products were purified and sequenced. Results Suzhou clinical isolates of Escherichia coli and Klebsiella pneumoniae strains producing ESBLs detection rates were 64.7% and 60.5%. ESBLs strains to produce third-generation cephalosporin ceftriaxone, cefotaxime, ceftazidime 41.9% ~ 86.3% to ciprofloxacin, levofloxacin resistance rate of 48.4% ~ 85.5%, for piperacillin / tazobactam, cefoperazone / sulbactam was 4.2% ~ 25.8% for imipenem and meropenem 100% sensitive. PCR and sequencing showed ESBLs producing strains, β-lactamase TEM-type gene 61, CTX-M13-type 55, CTX-M1-type 2, OXA1 type 19, SHV-type 15. Conclusion Suzhou clinical isolates of Escherichia coli and Klebsiella pneumoniae strains producing ESBLs detection rate and to cephalosporins and quinolones resistance rates reached a high level, should strengthen the clinical monitoring of ESBLs strains ; Suzhou City clinical isolates of ESBLs-producing Escherichia coli and Klebsiella pneumoniae β-lactamase genotype TEM and CTX-M13 main type; imipenem and meropenem treatment of ESBLs-producing Escherichia coli and Klebsiella pneumoniae infection drug of choice.

The Analysis of Current Situation of Bacterium Resistance to Antibiotic in Suzhou and the Detection of Partly Antibiotic-resistance Bacterium and Their Genotype Distribution

Abstract

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