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Study on Digestive Enzyme-producing Bacteria in the Intestinal Tract of Ctenopharyngodon Idellus and Carassius Auratus Gibelio

Author: FengXue
Tutor: ChenXiaoZuo
School: Huazhong Agricultural University
Course: Hydrobiology
Keywords: Grass carp Silver crucian carp Bacillus Intestinal flora Digestive enzymes
CLC: S917.4
Type: Master's thesis
Year: 2008
Downloads: 189
Quote: 1
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Abstract


This test to grass carp (Ctenopharyngodon idellus) and silver crucian carp (Carassius auratus gibelio) test, a grass carp and silver crucian carp intestinal digestive enzymes secreted extracellular bacteria and sieve yield enzyme activity from the intestinal Bacillus grass carp feed at different concentrations, the research activity Bacillus grass carp intestinal flora and intestinal and liver pancreas digestion. Grass carp and silver crucian carp intestinal digestive enzymes in bacteria secrete extracellular trials, detection of protease, lipase, amylase and cellulase enzyme production capacity of 180 bacteria were isolated from the intestinal tract of grass carp and silver crucian carp . The results show that the two fish gut digestive enzymes in bacteria, including Aeromonas (Aeromonas), Vibrio (Vibrio), Bacillus (Bacillus), Pseudomonas (Pseudomonas) four species of bacteria secrete extracellular Aeromonas dominant in which 45.71% of Aeromonas secreted extracellular digestive enzymes. Grass carp digestive enzymes strains can secrete the four extracellular total of 33, accounting for 36.67% of the total number of intestinal bacteria; 43 silver crucian carp, accounting for 47.78% of the total number of silver crucian carp intestinal bacteria. On the distribution of enzyme production strains, grass carp production of intestinal digestive enzymes the number of bacteria significantly more than foregut and hindgut (P <0.05), before, during and after intestinal 6, 20 and 7; silver crucian carp midgut and hindgut enzyme production bacteria count was no significant difference the foregut distribution least. Grass carp secretion of protease, lipase, amylase and cellulase strain were 21 (23.33%), 10 (11.11%), 30 (33.33%) and 16 (17.78%). Silver crucian carp is not detected in the intestinal bacteria that secrete cellulase, protease, lipase and amylase strains 21, 37 and 17. Test research Bacillus Ch9 grass carp intestinal flora, the groups feed additive Bacillus Ch9 concentration of 1.0 × 10 9 cfu / kg feed (F group) group are 3.0 × 9 cfu / kg feed (F 3 ), 5.0 × 10 9 cfu / kg feed (F 5 group) and do not add (F 0 group). The feeding cycle 56d, test every 14d sampled once, were measured quantity, type and proportion of grass carp intestinal aerobic and facultative anaerobic bacteria and anaerobes. The results show: (1) feeding Bacillus Ch9, the number of bacteria in the intestines of grass maintained at 10 6 -10 7 cfu / g, between the two groups had no significant difference ; (2) after feeding Bacillus Ch9 grass carp intestinal gas Aeromonas bacteria Enterobacter (Enterobacteriaceae) the proportion of the increase in the number of days with the Bacillus ration and feeding lower proportion of Bacillus significantly higher . The total amount of intestinal anaerobes and Bifidobacterium (Bifidobacterium), the number of lactic acid bacteria (Lactobacillus) since the addition of Bacillus increase, Bacteroides number of (Bacteriodes) there was no significant change. In in research Bacillus Ch9 digestive enzymes on the intestinal Hepatopancreas test, the basis of the same grain were added to 1.0 × 10 9 cfu / kg feed (of F 1 < / sub> group), 3.0 × 10 9 cfu / kg feed (F 3 group), 5.0 × 10 9 cfu / kg feed ( F 5 group) and Bacillus add (F 0 group), feeding an average weight of about 50g of grass carp, different parts of the study of the intestinal, liver and pancreas protease, amylase, lipase activity. The results show that: Bacillus Ch9 in the short term (14-28d) can enhance the grass carp (front, middle, hindgut) and hepatopancreas protease, amylase, lipase activity, prolonged feeding (42d- 56d) the activity of digestive enzymes no longer rise, but still higher than the normal level. This indicates that: appropriate dose of Bacillus Ch9 to improve grass carp intestine and hepatopancreas digestive enzyme activities, promote digestion and absorption of the grass carp prey, while the long-term feeding will not further enhance the role of the digestive enzymes.

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CLC: > Agricultural Sciences > Aquaculture, fisheries > Aquatic basic science > Aquatic Biology > Aquatic Zoology
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