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Background: China Hepatitis B high incidence of hepatitis B virus (HBV) infection by up to 9.75%, especially in southern China is staggering, Guangdong, Guangxi, Hainan crowd of hepatitis B surface antigen (HBsAg) The positive rate of 12% to 17%. HBV infection, family aggregation attract scholars to study the vertical transmission of HBV, which after the possibility of germ cells spread to the next generation, as well as HBV sperm is an important research direction. Detected HBV DNA from semen, sperm and egg cells have been reported in the state of integration of HBV DNA found in the sperm, but its exact form of existence, the mechanisms of pathological significance and epidemiological significance are yet to be elucidated. In recent years, assisted reproductive technology (ART) is generally carried out in major cities of the country, the infertile couples HBsAg positive rate is not lower than the general population, chronic HBV infection is not a contraindication of ART. Important route of transmission as HBV vertical transmission of mother-to-child transmission has been a series of studies, confirmed and recognized, and has had a better preventive measures, the man sperm and in vitro fertilization - embryo transfer (IVF-ET) treatment of chronic HBV infection at home and abroad reported rarely. HBV Can semen artificial insemination (AI) infected woman, contain or integration of HBV DNA in sperm in IVF-ET process of fertilization, embryo implantation, embryonic development may affect should be enough to cause the field of reproductive medicine attention. Objective: to understand HBV whether infected sperm; through quantitative detection of male patients with chronic HBV infection, serum, seminal plasma, sperm of HBV DNA to investigate the possible correlation between of three specimen HBV infection status and viral load. 2 man with chronic HBV infection, in vitro fertilization - embryo transfer outcome. Method: a collection of blood and semen samples, strict separation of seminal plasma and sperm by fluorescence quantitative PCR (FQ-PCR) technology quantitative detection research group of 58 cases of male patients with chronic HBV infection and the control group, eight cases of hepatitis B virus markers ( the HBVM) negative serum, seminal plasma and sperm HBV DNA analysis of the differences in the quantitative results, may affect blood of HBV sperm. Woman man HBsAg positive HBsAg-negative infertile couples 132 for the study group, the two sides HBsAg were negative infertile couples 264 pairs for the control group, the two groups were compared cumulative embryo score (CES), fertilization rate, egg crack rate, quality embryo rate, implantation rate, clinical pregnancy rate, the rate of early abortion, late abortion rate, delivery rate and neonatal malformation rate. Study Group 1 serum HBV DNA positive rate was 39.7% (23/58), the average quantitative value of 8.04 × 10 6cp/ml seminal plasma HBV DNA positive rate was 12.1% (7/58), the average quantitative value of 1.62 × 10 ~ 5cp/ml, sperm HBV DNA positive rate was 8.6% (5/58), the average quantitative value 51.07cp/10 ~ 5 cells (2.41 × 10 ~~ 4cp/m1); 5 patients, serum, seminal plasma, sperm HBV DNA positive seminal plasma and serum HBV DNA-positive patients, but the sperm HBV DNA-negative, 16 patients seropositive seminal plasma, sperm HBV DNA were negative, 35 patients with serum, seminal plasma, sperm HBV DNA were negative. Eight cases of the control group, serum, seminal plasma, sperm HBV DNA were negative. 2 serum, seminal plasma, sperm HBV DNA level significant difference at (χ ~~ 2 = 43.053, d_f = 2, P = 0.000), serum highest viral load, the amount of seminal plasma virus Secondly, virus lowest sperm . Sperm, seminal plasma, serum level of HBV DNA test results twenty-two there are positive correlation (P lt; 0.05). 3132 man HBsAg positive group and 264 cases of male cumulative embryo score HBsAg negative group (51.7 ± 18.1, 55.1 ± 16.5), fertilization rate (68.3% vs. 65.8%), cleavage rate (96.9% vs. 96.9%), quality embryo rate (33.5% vs. 35.1%), implantation rate (37.5% vs. 35.0%), clinical pregnancy rate (53.0% vs. 49.2%), early abortion rate (5.7% vs. 4.5%), late abortion rate (7.1% vs. 3.7 %), farrowing rate (39.4% vs. 46.2%) and neonatal malformation rate (0, 0), between the two groups of data is very close to (P gt; 0.05). Conclusions chronic HBV infection seminal plasma, sperm of HBV DNA; 2 serum HBV DNA level is the higher, the easier detection of HBV DNA in the sperm; 3 man with chronic HBV infection does not affect the treatment outcome of IVF-ET;
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