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Within the multiple advantages of this study, based on the realities of the current influenza epidemic status and more than one subtype of highly pathogenic avian influenza virus interspecies transmission , the successful application of bioinformatics theory screened influenza virus epitopes ( influenza virus conservative M the NP proteins CTL epitopes , and H1 , H7 , H9 HA Th cell epitopes ) , design and synthesis of the common for more than one subtype of influenza A virus multi-epitope gene cassette Epi I Epi II. The DNA recombinant pVAX1- to pVAX1 eukaryotic expression plasmid vector was constructed and screened a total of express NP Epi I gene cassette the DNA recombination body pVAX1-NP-Epi I, H5, H3 subtype IV HA Epi II gene co-expression H5HA-Epi II-H3HA1-M2e. Applications BALB / c mice animal models for immunogenicity preliminary study , the the immune results showed that recombinant DNA vaccines induce the production of anti-influenza H1 , H3 , H5 , H7 and H9 HA humoral and cell-mediated immunity , which induced the H5HA , H3HA1 higher antibody levels ; lymphocyte transformation test lymphocyte sub- class the FACS detection and γIFN-ELISPOT cellular immune indicators detect recombinant multi-epitope induced strong cell-mediated immunity , indicating that the recombinant DNA construct good immunogenicity , laid the foundation for the final multivalent vaccines can prevent a variety of subtypes of influenza virus .
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