|
Objective: To investigate Jinkuishenqiwan reproductive function in male SD rats intraperitoneal injection of cyclophosphamide (CTX) and part of the mechanism of action . Methods: The 15 -month-old male SD rats were randomly divided into four groups : model group , Jinkuishenqiwan low-dose group , middle dose group and high dose group , each group of 10 . Each group of rats were given intraperitoneal injection of cyclophosphamide suspension (1ml/kg ยท d -1 , 1 / d) for 5d; model group to distilled water , Pill low dose group , middle dose group and high dose groups , respectively to give Jinkuishenqiwan suspension low dose , medium dose , high dose gavage for 28 consecutive days . Observe and record daily activities in rats , body weight , and changes in mental status ; 24 hours of the last administration, the rats were sacrificed , measured testicular epididymis wet weight ; take epididymal fluid microscope sperm density , vitality and viability ; optical mirror image analysis system to observe the microscopic structure of the testis ; measured serum testosterone ( T ) . Results: from the first four days of the owner-occupied cyclophosphamide , the rats in each group showed weight loss, reduced activity, unresponsive body hair curly , yellow , dry dull easy to fall off , tail clammy kidney yang - like symptoms : while gold Kui Shen Qi Wan each group can improve the symptoms and performance ; the end of the experiment , the treatment group compared with model group , organ coefficient in the testis epididymis , sperm concentration , sperm motility and viability , testicular seminiferous tubule diameter and interstitial cell number serum testosterone are improved statistically significant. Conclusion : Cyclophosphamide exposure can copy the SD rats deficiency and reproductive dysfunction model , resulting in decreased reproductive capacity in rats , Jinkuishenqiwan can improve organ coefficient of cyclophosphamide in rats exposed to testicular epididymis ; improve cyclophosphamide exposure the rat semen sperm density , vitality and survival rate ; improve cyclophosphamide number of rat testis seminiferous tubule diameter and interstitial cells exposed to ; improve cyclophosphamide in rats exposed to serum testosterone levels . Come Jinkuishenqiwan can effectively antagonize reproductive toxicity of cyclophosphamide SD rats , and protect their reproductive function .
|