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Study of the body 's metabolism of the active ingredient in rat of Chuanxiong Xiaoxuming soup

Author: DingChunGuang
Tutor: ZhangJinLan；MaChen；HeWenYi
School: Peking Union Medical College , China
Course: Drug analysis
Keywords: Chuanxiong Volatile oil Ligustilide Metabolites Cytochrome P450 Xiaoxuming soup High Performance Liquid Chromatography High Resolution Mass Spectrometry LC-MS
CLC: R285.5
Type: Master's thesis
Year: 2008
Downloads: 247
Quote: 1
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Abstract


Chinese medicine and its compound is a treasure of the Chinese nation, is an important source of independent research and development of innovative new drugs. The herbal compound pharmacokinetics study helps to reveal the material basis and mechanism of action of the compound pharmacodynamic of great significance for the modernization and internationalization of Chinese medicine. Selection of the papers Chuanxiong single flavor herbs and Xiaoxuming, soup as an object of study, the rat oral medicine extracts and monomer components after monomer components in the metabolic characteristics of rats and rats after oral administration of traditional Chinese medicine, extracts and active ingredients group after some of the active ingredients pharmacokinetic differences exploratory research, Chinese medicine pharmacokinetic study provides reference and ideas. Chuanxiong is one of the well-known traditional Chinese herbal medicine, only contained in the \wind musculoskeletal, bone-setters throat and other diseases. This paper compares the rats were orally Chuanxiong volatile oil and its main components in the ligustilide ligustilide differences in the metabolic characteristics of rats of the metabolites ligustilide structure identification, study and explore volatile oil of different subtypes CYP450 enzyme inhibition. The paper established a reversed-phase high-performance liquid chromatography of the rat oral Chuanxiong volatile oil and ligustilide analysis of urine, fecal bile metabolites. Chromatographic conditions: Phenomenex C 18 (4.6 × 250 mm, 5μm) column, mobile phase gradient elution of acetonitrile and 0.1% acetic acid aqueous solution at a flow rate of 0.8 mL / min, detector diode array detector (DAD), wavelength scanning, detection wavelength 280 nm, the column temperature was 25 ° C. The results showed that the rats were orally volatile oil and monomer ligustilide ligustilide metabolites in rats from the urine, fecal metabolites containing a small amount of the original drug, was not detected in the urine and feces . Bile metabolites less high content of the original drug. But it is noteworthy that the rat oral volatile oil the metabolites ligustilide after both the number and the relative content of larger differences with oral ligustilide monomer, indicating that the other ingredients in the volatile oil of this lactone main ingredient Wara metabolism may have a certain impact. 7 metabolites from rat urine detected isolated six metabolites identified, according to the NMR, MS and MS / MS data of which the structure of the four metabolites, respectively the Chuanxiong lactone I (senkyunolide I), 3, 5 - hydroxy-3 - butylphthalide (3,5-dihydroxy-3-butylphthalide), 6 - hydroxy-7 - mercaptomethyl Ligusticum lactone (6-hydroxy-7-sulfmethyl ligustilide), ligustilide C (senkyunolide C). The experimental results provide important reference data for ligustilide pharmacokinetics studies and clinical applications. This thesis volatile oil vitro inhibition of enzyme CYP4501A2, 2C9, 2C19, 2D6, 2E1, 3A4, etc., the volatile oil According to the projections of enzyme CYP4501A2, 2C9, 2C19, 2D6, 2E1, 3A4, etc. IC 50 are much larger than 10μM, showed no inhibition, to provide a theoretical basis for the combination of clinical safety. Pharmacokinetic characteristics of the the Xiaoxuming soup, HPLC-MS methods rats were oral the extract of soup total of the soup effective ingredients Xiaoxuming group and Xiaoxuming, 5-O-methyl 基维斯阿米 alcohol and liter Ma Su concentration in rat plasma. Sample preparation using ethyl acetate liquid - liquid extraction, internal standard selection phenacetin, column: InertsilODS-3 (2.1 mm × 100 mm, 3/μm), a mobile phase of acetonitrile and 0.1% aqueous formic acid system ( 30:70, v / v), flow rate of 0.2 mL / min, column temperature: 30 ° C; linear ion trap mass spectrometer, ESI ion source, using full-scan positive ion monitoring mode, monitoring the mass number of 5-O-A the 基维斯阿米 alcohol is m / z 290.5-291.5; cohosh prime m / z 306.5-307.5; internal standard phenacetin m / z 179.5-180.5 5-O-A 基维斯阿米-alcohol of Cimicifuga hormone in plasma linear range were 11.5-230.0ng/mL (r> 0.99) ,10.4-936 .0 ng / mL (r> 0.99). Day precision and intra-day precision RSD were less than 10.7%. The results showed that the 5-O-methyl group of the active ingredients of the the rat oral Xiaoxuming soup 基维斯阿米 alcohol peak speed in the plasma concentration-time curve of 5-O-methyl 基维斯阿米 alcohol cohosh prime slower in oral total extract (T max (min): 480 versus 360; C max (ng / mL): 338.03versus 318.46). Oral Xiaoxuming soup ingredients group Cimicifuga hormone peak speed faster than oral total extract (T max / (min): 180 versus 420; C , max ( ng / mL): 2018.33 versus 1625.73). The results showed that the total extract-Africa active ingredient has a certain effect on the pharmacokinetics of the active ingredient group dynamics. The experimental results for a more in-depth study of Xiaoxuming soup pharmacokinetic characteristics in rats provide scientific data, and provide important reference and research ideas for the pharmacokinetic study of traditional Chinese medicine on.

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