Dissertation > Excellent graduate degree dissertation topics show

Study on the Secondary Metabolites of Two Fungi from the South China Sea

Author: XiaoBiHong
Tutor: LeiXiaoLing
School: Guangdong Ocean University
Course: Aquatic Products Processing and Storage Engineering
Keywords: marine fungi secondary metabolites algae fungi coral fungi biological activity
CLC: R284
Type: Master's thesis
Year: 2011
Downloads: 19
Quote: 0
Read: Download Dissertation


The complex and changeable ocean’s environment has caused the marine microorganism’s multiplicity. The marine microorganisms because of their special survival environment and metabolism way, have been produced much novel secondary metabolites with novel skeleton .These compounds have demonstrated antitumor,antibacterial,antifungal or other bioactivities, which have provided the new hope for the new medicine development and difficult disease cure.The metabolites of seaweed endophytic fungi ZJ27 were studied in this master’s thesis and ten compounds were isolated. The structures were identified by comprehensive physic-chemical properties and spectral methods as sclerotiorin (2-1),ochrephilone (2-2),6-((3E,5E)-5,7-dimethyl-2-methylenenona-3,5-dienyl)-2,4-dihydroxy-3-methylbenzaldehyde (2-3) ,( 2E,4E ) -4,6-dimethylocta-2,4-dienoic acid ( 2-4 ), pencolide ( 2-5 ) , 1,3,8-trihydroxy-6-methyl-9,10-anthraquinone(2-6), physcion(2-7), emodindi- methylether(2-8), emodin(2-9), chrysophanol(2-10). Among them, compounds 2-1、2-2、2-3 were structural unique and compounds 2-6、2-7、2-8、2-9、2-10 were series of benzene quinone compounds.The metabolites of Galaxea fascicularis endophytic fungi XW4-12 were studied in this thesis and eleven compounds were isolated. The structures were 3-[[2,3-dihydro-4,5-bis (3-methyl-2-buten-1-yl) -2-(1,1-dimethyl-2-propen-1-yl)-1H-indol-3-yl]methyl]-2,5- piperazinedi(3-1)、3,3’-diisopropyl-2,3,5a,6,11,11a,2’,3’,5’a,6’,11’,11’a-dodecahydro- [10b, 10’b]bi [pyrazino[1’,2’:1,5] pyrrolo [2,3-b] indolyl]-1,4,1’,4’-tetraone(3-2)、1,8-dihydroxy- 6-methoxy-9,10-dioxo-9,10-dihydroanthracene-3-carboxylic acid(3-3)、cyclo-(Pro-Gly)(3-4)、cyclo-( Leu - Pro)(3-5)、mannitol(3-6)、pyridin(3-7)、ergosterol(3-8)、7,22(E)-ergostadiene-3β,5α,6β-triol(3-9)、7,22-(E)–diene-3β,5α,6β-triol-ergosta(3-10)、3,5-dihydroxy ergosta-7,22-diene-6-keteon(3-11)。And compounds 3-1、3-2were structural unique and compounds 3-8、3-9、3-10were series of sterol compounds. Besides, it reported the NMR data of compound 3-2 for the first.Meanwhile,the study was about antibacterial activity and enzyme inhibition activity of these compounds. The study determined their inhibitory activity of bacteria and fungi by the 96-well plate method and growth rate method respectively. Compound 2-1 had remarkable inhibition of Bacillus subtilis, Staphylococcus albus and Sarcina luteus, and the MIC were 0.1, 0.2, 0.2 mmol/L. Compounds 2-1 and 2-2 had notable inhibition of Aspergillus niger, Fusarium oxysporum, Rhizoctonia solani and Candida albicans, which the IC50 of compound 2-1 was 15.06、3.09、3.68、8.19 mmol/L and that of compound 2-2 was 22.28、21.32、8.18、21.75 mmol/L. Compounds 2-2, 3-5 had inhibition of cholinesterase and the IC50 were 0.0905、0.0985 mmol/L. Compounds 2-2、2-3、3-5、3-3 had inhibition ofα-glycosidase and the IC50 were 0.0868、0.1049、0.1013、0.1009mmol/L. Compounds 2-1、2-2、2-3、2-4、3-3 had inhibition of DNA topological I and the IC50 were 0.0868、0.1049、0.1013、0.1009 mmol/L.The master’s thesis was about the metabolites of marine fungi ZJ27 and XW4-12 and their activity, which results showed that marine fungi not only contained rich compounds, and most of the compounds had the good antibacterial activity and enzyme inhibition activity. Therefore, they may develop to be medical source microorganism.

Related Dissertations

  1. Endophytic Fungal Diversity and Their Secodary Metabolites of Eleven Dominant Plant Species in the Dry-hot Valley of Yuanjiang, Yunnan Province, China,X172
  2. The Antioxidative Efficient Component Recognition for Curcuma Longa L. Based on the Quality Control Pattern of Constituent Knock-out/Knock-in,R285
  3. Medical magnesium alloy surface laser cladding Zr 60 Ti 6 Cu 19 Fe 5 Al 10 amorphous coating,TG174.44
  4. TC4 alloy pulsed laser deposition of hydroxyapatite microstructure and properties of,TG146.23
  5. Pichia Isolation, purification and biological activity,Q936
  6. Colostrum PRPs features and functional studies,TS252.1
  7. Preparation of hollow spheres of titanium dioxide and biological activity,TB43
  8. Synthesis and Biological Activity of A Novel Non-steroidal Anti-inflammatory Drug ML4000,R96
  9. Expression of Human Nerve Growth Factor in Cho Cells with Various Vectors,Q42
  10. Study on Municipal Wastewater Treatment Technology for Further Nitrogen and Phosphorus Removal Basing on Composite Ferric Enzymatic and Composite Filler Biofilm,X703.1
  11. Study on the Extraction of Natural β-Carotene and the Development of Its Soft Capsule Product,R151
  12. The Research of the Synthesis of Ferroceneyl Deriveatives by Suzuki-Miyaura Reaction and the Synthesis of 1, 3, 4-Oxadiazoline,O626
  13. Effects of Long-Term Continuous Cropping on Cotton Growth and on Cotton Field Soil Biological Activity,S562
  14. SiO_2 glass of calcium phosphate crystal phase composition and biological activity,R318.08
  15. Two actinomycetes of the genus Streptomyces active Secondary Metabolites,R91
  16. Effectiveness of Long-lasting Insecticidal Nets Washed with Non-standard Washing Procedure,R184.31
  17. Preparation of Luidia Quinaria Von Martens’ Main Active Substances and Research on the Activity of Luidia Quinaria Von Martens’ Acidic Mucopolysaccharide,R151
  18. Effects of Individual Copper, Zinc, Lead and Cadmium Pollution on Biological Activities of Aquic Brown Soil,X53
  19. The Synthesis, Structures and Fluorescent Properties of Complexes Containing Indole-3-Acetic Acid Ligand,O621.13
  20. 2 - aryloxy methyl benzimidazole derivatives microwave synthesis, biological activity and Crystal Structure,O626.23

CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Traditional Chinese medicine chemical
© 2012 www.DissertationTopic.Net  Mobile