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The Chlamys farreri occupies an important position in the mariculture industry in China, bring huge economic benefits to our country in northern coastal areas. But since the 1990s, large-scale Chlamys farreri death in northern China breeding sea year after an outbreak of severe economic losses caused irreparable. Previous studies have shown that, AVNV Chlamys farreri late in the culture of a large number of deaths directly pathogenic, but about the pathogenic popular mode of transmission remains unclear. In order to research the plankton to carry AVNV possibility of the papers FQ-PCR detection technology, from 2008-2009 2 Qingdao Shazikou and Rongcheng Sanggou, Bay scallops breeding sea the many months plankton and macroalgae to carry AVNV the quantitative detection, the aim of to clarify its seasonal variations in plankton and macroalgae to carry the AVNV the to provide a theoretical basis for understanding the spread of AVNV and develop prevention and control measures. This study from May 2009 to November, respectively, from Qingdao, Shazikou and Rongcheng Sanggou, Bay scallop breeding sea regularly water samples taken 50cm and 300cm two water layer, 25μm, 3μm, three 0.22μm pore size filter The film classification three different particle size of plankton was collected by filtration, quantitative detection using quantitative PCR technique the plankton to carry AVNV the situation. The results showed that the the two sea plankton samples detected AVNV, number of AVNV detection also peak in August, sea Shazikou per liter of seawater plankton carrying 4.11 × 106 copies AVNV, Sanggou Bay the sea plankton carries 1.49 × 105 copies AVNV. The three particle the plankton components carry AVNV the number of low to high particle size of 0.22μm-3μm component, 3μm-25μm particle size component and greater than 25μm particle size component. But two of the aqueous layer (50cm and 300cm) plankton carry AVNV quantity is not a significant difference. This result suggests and the breeding sea the plankton can carry AVNV, carry AVNV number significant seasonal variations, different sizes of plankton carry a significantly different number of AVNV. Characteristics of the scallop feeding plankton, the results of this study show that the scallop-pass filter in the breeding period planktonic food food-borne viruses and the infection AVNV possible, sea plankton may be important cause AVNV popular media. 2008 - 2009 14 months from Qingdao Shazikou sea randomly collected 10 species of large algae (Ulva pertusa Uiva PERTUSA ON, of Enteromorpha prolifera Enteromophites the Sea film Halymenia floresia the fuzz agar Gelidium crinale, edge tube of Enteromorpha Linza Enteromorpha the tongue shaped filicina Grateloupialivida, the flat Gracilariaceae Gracilaria textorii section folder algae in Lomentaria Lyngb, Cladophora SPATIAL AND TEMPORAL Sargassum Sargassum) species (Ulva pertusa Uiva PERTUSA ON, of Enteromorpha prolifera Enteromophites, the Sea film Halymenia floresia fuzz Gelidium Gelidium crinale, the edge tube enteromorpha Enteromorpha Linza flat Gracilaria Gracilaria textorii Cladophora Cladophora) detected the virus, infected samples accounted for 55.56% of the total number of test samples, 12 of Ulva pertusa samples 11 samples detected virus Ulva pertusa samples collected on August 25, 2009 to carry the highest amount of virus, the 7.68 × 104/0.1g. 14 kinds of large-scale seaweed samples collected from Rongcheng Sanggou Bay sea folder (Ulva pertusa Uiva PERTUSA ON, of Enteromorpha prolifera Enteromophites, the Sea film Halymenia floresia the fuzz agar Gelidium crinale, the centipede the algae Grateloupia livida of tongue-shaped section algae Lomentaria Lyngb, Mawei algae Sargassum, the Gracilaria Gracilaria confervoides, tine algae Gymnogongrus flabelliformis the Gelidium Gelidium amansii, the Gracilaria lemaneiformis of asparagus, gold film the algae Chrysymenia Wrightii, centipede the algae Grateloupia filicina, kelp, Laminaria of Japonica) 3 species (Ulva pertusa Uiva pertusa sea ??film Halymenia floresia, Sargassum Sargassum) detected viruses, infected samples accounted for 20.83% of the total number of test samples of Ulva pertusa samples collected in July 2009 carrying the virus the highest number of 3.06 × 103/0.1g . This result suggests that part of macroalgae can carry the AVNV, macroalgae to carry AVNV role in the epidemic spread of the pathogen remains to be further studied. 9 Microalgae laboratory culture analysis scallop breeding sea plankton species and abundance of eukaryotic control, PCR amplification of eukaryotic plankton of the nine month Qingdao Shazikou scallop breeding sea area 18SrDNAV1-V3 hypervariable area, and using the the DGGE technique of amplified fragment analysis, the results showed that the amplified sequence length 560 bp DGGE analysis of different algae species can be separated. DGGE analysis of eukaryotic microbial species diversity Shazikou breeding nine months, the results from the nine month 2 meters deep seawater in total amplified 38 classes bands, of which 3 bands total of nine months, the total number of 7.69%, the characteristic bands of different month 11, 28.2% of the total number of bands. The number of bands in different months watery significantly different, the Shannon diversity index of 0.4632. The similarity analysis showed that in March 2009 and April 2008 and June 2008, 10 and December breeding sea eukaryotic microbial species similar to. UPGMA cluster analysis based on the types of micro-organisms in water samples of different months, the results show that the 2008 1,2,3,4 the 5,6,8 month and 2009 are clustered into a display similar to the sampling time of the month microbial species similar highest.
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