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In this study, using FIASCO ( Fast Isolation by AFLP of Sequences COntaining repeats ) method were constructed variegated abalone (Haliotis diversicolor) (CT) n microsatellite enriched library and large yellow croaker (Pseudosciaena crocea) (AC) n microsatellite enriched library , and get a lot of microsatellite sequences , developed a new tag . Variegated abalone ( CT ) n microsatellite enriched library contains 1212 clones , to reap 114 cloned and sequenced , 48 microsatellite sequences positive clones was 40.5% . 11 pairs of polymorphic microsatellite markers developed from these microsatellite sequences . 24 wild Haliotis diversicolor individuals detect the polymorphism of the 11 mark , the number of alleles ranging from 3-10 , polymorphic information content (PIC) of 0.148 to 0.818 , observed heterozygosity of 0.083 ~ 0.913 0.159 to 0.856 . 9 sites , expected heterozygosity met with balance , the bit point the SA-JMU4 and the SA-JMU12 apparent deviation from the Hardy - Weinberg equilibrium ( P lt; 0.01 ) . Large yellow croaker (AC) n microsatellite enriched library contains 1440 clones , 257 clones were sequenced to reap 182 clone containing the number of repeat ≧ 10 microsatellite sequences , positive clones was 70.8% . From 26 pairs of primers designed to detect the polymorphism of the 10 sites , 3 to 12 the number of alleles , polymorphic information content of 0.283 to 0.866 and 0.233 to 0.900 , observed heterozygosity , expected heterozygosity is 0.326 to 0.893 . Found the existence of possible chain of sites , but in addition to LYC0044, LYC0046, LYC0053 and LYC0060 four points , the remaining sites are deviated from Hardy - Weinberg equilibrium . Papers from a technical point of the aquatic animals microsatellite enriched library building , improve microsatellite markers yield ; Haliotis diversicolor large yellow croaker wild population genetic polymorphisms problem was also discussed . In this study, the newly developed microsatellite markers most PIC values ??higher than 0.5 , applicable to a variety of genetic analysis , their presence will help promote the the Haliotis diversicolor large yellow croaker research further depth .
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