Dissertation > Excellent graduate degree dissertation topics show

PSGMS a physical map of the rice genome pms1 build and candidate genes determine

Author: LiuNan
Tutor: ZhangQiFa
School: Huazhong Agricultural University
Course: Biochemistry and molecular biology
Keywords: pmsl BAC clones Physical map cDNA
CLC: S511.03
Type: Master's thesis
Year: 2000
Downloads: 235
Quote: 4
Read: Download Dissertation

Abstract


PSGMS rice performance under long- day conditions , male sterility, fertility restoration under short- day conditions . Hybrid Seed heading and the corresponding male parent varieties with group (Restoration) under long- day conditions , can be used as a sterile line; heading under short-day conditions , but also self- seed , used as a maintainer line , Department of dual-use is important germplasm resources to achieve the two-line hybrid seed production . Studies have shown that either Indica or Japonica PGMS rice chromosome 7 major infertility gene pmsl . This study is , build to completed PSGMS physical map of rice gene pmsl section in accordance with the technical route map-based cloning , the entire physical map consists of eight BAC clones composed the span of about 500kb ; gene recombination events on both sides of the pmsl determine a 115kb BAC clone ; build MH63 young panicle cDNA library screening, and access to an insert size of approximately 1.8kb of cDNA clones . Speculated pmsl gene candidate genes , which lay a solid foundation. The main results are summarized as follows: 1. Building a photosensitive genic male sterile rice the pmsl section physical map . The entire physical map composed by 8 BAC clones span of about 500KB . 2. Gene recombination events on both sides of the gene is located on a 115kb BAC clones . 3. Each gene on both sides to find a polymorphic SSR markers gene apart 0.25cM . 4. Select the the MH63 from panicle initiation Ⅲ to Ⅴ period of inflorescence , hybrid cDNA library was constructed , the average insert size of 1.4 kb . And to prove the integrity of the library better . 5. Filter , and to obtain an insert size of approximately 1.8kb of cDNA clones . 6. BAC clone 21O9 10 expressed sequence of period -specific expression of the young spike .

Related Dissertations

  1. Analysis of Infrared Spectral Features and Identification of Al-responsive Genes in Small Black Soybean with Aluminum Stress,S529
  2. Molecular Cloning, Prokaryotic Expression and Characterization of a Novel Trypsin Inhibitor in the Frog, Lepidobatrachus Laevis,Q78
  3. Construction and Primary Analysis of Differentially Expressed cDNA Library of Alfalfa by Using Suppression Subtractive Hybridization under the High Temperature,S541.9
  4. The cDNA-AFLP Analysis of Gene Differential Expression and RNA Editing of ATP6 Gene between Cytoplasmic Male Sterile Lines and Their Maintainer Lines in Soybean (Glycine max (L.) Merrill),S565.1
  5. The Genes Expression Analysis of Cotton Fiber Initiation Stage and Characterization of Three New Genes in Gossypium,S562
  6. Construction and Analysis of a Subtractive cDNA Library on Early Embryonic Development Related Genes in Ducks,S834
  7. Establishment of Feline Calicivirus (FCV) Reverse Genetics System Using RNA Polymerase Ⅱ and Expression of RHDV VP60 Gene,S852.65
  8. Studies on Cytoplasmic Male Sterility and Restoration in Cotton (Gossypium Hirsutum L.),S562
  9. The DNA Cloning and RNA Interference of Some Functional Genes in Laodelphax Striatellus (Fallen),S435.112.3
  10. Studies on Stress Resistance, cDNA Microarray and Carbohydrate Metabolism in Tomato Seedlings under Low Night Temperature,S641.2
  11. Cloning and Expression of Novel β-Glucosidase Genes from Rhizopus Stolonifer Var. Reflexus,Q78
  12. Identification and Characterzation of Glutathione S-transferase Genes from Locusta Migratoria,S433.2
  13. The Complete Nucleotide Sequence and Infectious Clones of a Tomato Aspermy Virus Isolate,S432.41
  14. Molecular Cloning, Expression Analysis of Defence Related Genes from Citrus Infected by Ca. Liberibacter Asiaticus,S436.66
  15. Exploration and Functional Analysis of Olfactory Related Genes in the Lucerne Plant Bug, Adelphocoris Lineolatus (Goeze),S433
  16. Molecular Characterzation and Functional Study on Chitin Synthase Genes from Locusta Migratoria Manilensis (Meyen),S433
  17. Construction of an Full-Lengh Genomic cDNA Clone of Hev SAAS-JdY5,R346
  18. Screening for Protein Interacting with ALP1,Q943
  19. Cloning and Sequence Analysis of the CD109 Gene in Lamprey,Q78
  20. High Activty Cellulase Engineering Strain Construction by the Method of DNA Recombinantion,Q78
  21. The Model of Non-alcoholic Fatty Liver in Microtus Fortis Was Built and Researched,R575.5

CLC: > Agricultural Sciences > Crop > Cereal crops > Rice
© 2012 www.DissertationTopic.Net  Mobile