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Effect of Xiaotan Sanjie Recipe on Expresson of CAM in Gastric Carcinoma

Author: WangJianPing
Tutor: WeiPinKang
School: Second Military Medical University
Course: Traditional Chinese Medicine
Keywords: XTF Gastric Cancer Shift CD44 Carcinoembryonic antigen Intercellular adhesion molecule-1 E- cadherin
CLC: R273
Type: Master's thesis
Year: 2000
Downloads: 46
Quote: 0
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Abstract


Phlegm stasis is the production of tumor material basis Xiaotansanjie method is the treatment of tumors of the basic law, our department XTF developed into Jinlongshe oral achieved significant efficacy, the subject in previous clinical and experimental Based on findings from the cell adhesion mechanisms and invasion and metastasis start by XTF on cell adhesion molecule CD44, CEA, E-Cad, ICAM-1 expression, and to explore XTF anti-cancer invasion and metastasis mechanism. Preparation Xiaotansanjie medicated serum in vitro MKN-45 human gastric cancer cells, the proposed intervention XTF medicated serum cultured cells, MTT OD values ??measured to establish nude MKN-45 gastric adenocarcinoma ascites tumor model, Phlegm scattered Results square gavage to mice ascites tumor detection gall chamber organ metastasis nodules. Intravenously inoculated with cancer cells, the detection of lung metastatic nodules. ABC immunohistochemical method and the S-P assay gastric cancer cell adhesion molecule CD44, CEA, ICAM-1, E-Cad expression. Results: 1. In gastric cancer cells in vitro experimental observation, MTT assay Medicines OD value was significantly lower than the control group, the best inhibition rate was 34.35%. 2. Experimental observations in nude mice, tumor-bearing mice Medicines mesenteric tumor nodules and tumor weight were significantly lower than the control group, tumor-bearing mice Medicines number of lung metastases and tumor weight were significantly lower than the control group. 3. Medicines in gastric cancer cells E-Cad expression level was significantly higher than the control group, suggesting Xiaotansanjie can only enhance tumor cell membrane expression of E-Cad, thereby enhancing adhesion between cancer cells and prevent cancer cells from the original foci, inhibition of gastric cancer cell metastasis. 4. Medicines expression of CD44 in gastric cancer cells was significantly lower than the control group, suggesting Xiaotansanjie can only reduce tumor expression of CD44 on the cell membrane, thereby affecting cell adhesion and movement, thereby reducing the metastasis of tumor cells. 5. Medicines CEA expression in gastric cancer cells was significantly lower than the control group, suggesting Xiaotansanjie can decrease the expression of CEA on the tumor cell membrane, so that the heterogeneity of gastric cancer cell adhesion reduction, inhibition of tumor cell metastasis. 6. Medicines in gastric cancer cells the expression of ICAM-1 levels were significantly lower than the control group, suggesting Xiaotansanjie can only reduce tumor cell membrane expression of ICAM-1, thereby affecting cell adhesion and movement, thereby reducing the metastasis of tumor cells.

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