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On Electrophoresis Karyotype and Ribosomal DNA ITS-RELP of Fusarium Section Liseola

Author: LiuJinJie
Tutor: ZhengXiaoBo
School: Nanjing Agricultural College
Course: Plant Pathology
Keywords: The Leeser group Fusarium Mating group Mating type ITS region RFLP Electrophoretic karyotype Progenies Sexual offspring Colony color Growth rate
CLC: Q949.331
Type: Master's thesis
Year: 2000
Downloads: 139
Quote: 4
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Abstract


Transcribed spacer Leeser group Fusarium (Fusarium Section Liseola) for the experimental material, the study, carried out the following five aspects: (1) Lee Joseph Group Fusarium standard strains of different mating groups ribosomal DNA (rDNA) the District (ITS) restriction fragment length polymorphism (RFLP); (2) Leeser different mating type strain rDNA ITS-RFLP group Sickle the Mortierella A mating group (Fusarium moniliforme); (3) Leeser group sickle Mortierella A mating group strains of asexual and sexual offspring strains rDNA ITS-RFLP; (4) Leeser group sickle electrophoretic karyotype strains of Neurospora crassa A mating group; (5) Lee Joseph Fusarium A group mating group strains Determination of biological traits. Leeser group Fusarium rDNA ITS-RFLP study, using a pair of specific oligonucleotide primers ITS1 and ITS4 amplify in Leeser group Fusarium transcribed spacer of the mycophenolate rDNA about 620bp area (ITS), and optional Alu Ⅰ, Msp Ⅰ and Taq Ⅰ three kinds of identification the four bases restriction enzyme and restriction endonuclease digestion of the amplified product, the composition of the reaction system is as follows: the amplified product 8.8ul reaction buffer 1ul , restriction endonuclease 0.2ul, the reaction temperature in accordance with the use of each of the enzyme described. Digestion products using a 3% agarose gel electrophoresis after Popgene 1.31 software analysis, and the results are as follows: Lee Joseph, for the test group of six Fusarium mating group of 12 standard strains between the restriction fragment length polymorphism, similar analysis for the test strains can be clustered into two genetic similarity group, except F group positive and negative strains distribution in two genetic similarity group (45.5% similarity), the rest of the mating group standard strains distribution similar to the same genetic group, and the similarity is higher than 78.3%. Lee Joseph for the test group sickle Neurospora crassa A mating group strains rDNA ITS-RFLP similarity analysis clustering the two genetic similarity group, the similarity between the positive mating type strains higher, up to 100%; negative mating The similarity between the type strains is low, ranging from 34.8-100%. A mating group on female fertility strains asexual offspring ribosomal DNA (rDNA) ITS restriction fragment length polymorphism results show that asexual offspring of the same strain with each parent results for the test, indicating that in its asexual stability of the ITS region in the breeding process. The sexual offspring findings tested sexual offspring strains ITS region restriction fragment length polymorphism and the same as the parent strain, with only 38.1% of the male parent strains similarity. Studied by pulsed field gel electrophoresis electrophoretic karyotype studies, Leeser group sickle Mortierella female fertility strains electrophoretic karyotype electrophoretic better strains screened, and research electrophoretic karyotype in asexual and sexual offspring Yin were invited to pass. Using different electrophoresis conditions isolated the 6 $ chromosomal DNA of the tested strains bands, wherein D47 (parental strain), six bands of the order of 0. SMB, 2.53Mb, 3.25Mb 3 people 3Mb 3.80Mb the 4 stone OMb, molecular weight 18.16Mb; six bands of D52 (paternal strain) the size of the order of 1 * 3Mb 3.15Mb , 3.37Mb, 3 * SMb, 4.15Mb, 4.60Mb, the molecular weight of the electrophoretic karyotype 20.15Mb asexual progeny strains and their respective parental strains are identical, indicating karyotype can asexual progeny stable genetic; hybridization offspring strains electrophoretic karyotype is the same as the parent strain, with the paternal strain. Determination of number of biological traits in an experimental material determination of colony growth rate and colony color as the background material for this study. The results show that the growth rate of the asexual offspring strains tested, less colony color with the parent strain, but in sexual progenies strains, colony growth rate shows some differences, but colony color variation is quite large. The results of this study for phylogenetic analysis and genetic research The Leeser group Fusarium mold further laid the foundation, and provides the basis for some of the molecular level.

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CLC: > Biological Sciences > Botany > The plant taxonomy (system botany ) > Spore plants (implicit flowering plants) > Fungus > Fungi door > Imperfect Hymenomycetes
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