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Arbuscular mycorrhizal (AM) is widely distributed in nature, with more than 80% of the natural ecosystem vascular species symbiosis, the formation of arbuscular mycorrhizal. AM fungi to promote host plant mineral nutrient absorption, enhanced the resilience resistance to disease, have an important role in the maintenance of terrestrial ecosystems and plant diversity. AM fungi obligate symbiotic characteristics, in vitro pure culture has not yet been achieved. Therefore, AM fungi single bacteria culture is more than 40 years people have been exploring the key technology, but also the focus of the study of AM fungi and hot spots. Countries in the world are active in The AM fungi monoxenie culture research. This thesis cultured in vitro culture conditions substrate, metal ions, and environmental factors such as the AM fungal spore germination and germ tube growth and development of mycelium; legume milk vetch transformed by the use of hair root Agrobacterium (Agrobacterium rhizogenes) Ri T-DNA transformed roots and AM fungi Glomus intraradices, Gigaspora margarita, Gigaspora rosea to establish a dual training system. The ideal model of the dual training system is the study of AM fungal morphology, physiology, biotic and abiotic stress responses, rhizosphere biological interactions (PGPR, pathogens, nematodes) classification system evolution, it is now recognized as the only way to provide pure AM fungal material. Using this dual training system capable of AM fungi recognize each other legumes signal and symbiotic mechanisms. But this dual training system for the construction of AM fungi, legumes and rhizobia isolated triple culture system provides some reference. The papers main results are as follows: Gig. Margarita G. intraradices spores on water agar, MSR, M, MS medium could germinate on water agar germination and mycelial growth of the best. Low concentration of Zn2, La3 promote the gig. Margarita, Glomus intraradices spore germination, increased with the concentration, spore germination and mycelial growth inhibition. Agrobacterium rhizogenes K599 transformed soybean cotyledons scratch processing, and conversion of soybean root, but can not be grown in artificial media of low ionic concentration, it is of no use to build a dual training system with AM fungi. To the hair root hypocotyl Agrobacterium the K599 conversion of Chinese milk vetch, research different concentration of kanamycin transformed root induction rate, the result is: the Km concentration (40μg/ml) will increase the the milk vetch Ri T- The DNA conversion efficiency of the root is formed, while the high Km concentrations (60μg/ml) inhibition of the formation of transformed roots. Try to create a dual training system Gig. Margarita, Gig. Rosea, Glomus intraradices in MSR medium, and the three strains were transformed with the milk vetch root successfully established a dual training system. But the giant cyst spp GIG. Rosea fail to produce new spores. The reason may be that the species of giant cyst bacteria infect host mycelial growth lag phase exists, secondary spore production by exogenous mycelium biomass and the number of helper cells.
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