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Isolation and Studies on Some Biological Properties of Borrelia Burgdorferi from Cattle and Sheep in Xilingole Distrct of Inner Mongolia

Author: ZhaoHongMei
Tutor: WuNi
School: Inner Mongolia Agricultural University
Course: Preventive Veterinary Medicine
Keywords: Lyme disease Borrelia burgdorferi Isolation and identification of Biological characteristics
CLC: S852.61
Type: Master's thesis
Year: 2001
Downloads: 40
Quote: 0
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Abstract


The study in May 2000 , May , 2001 , from Xilinguole different grassland landscape with cattle and sheep body surface and grass collection prairie Haemaphysalis 1800 , using the direct fluorescent antibody method checks ticks carry diseases , the carrier rate averaged 7 .5 % (6 /80) . Darkfield microscope examination servant bacterial situation , the average infection rate of 2% ( 2 / 100 ) . Isolated from 520 prairie Haemaphysalis a Borrelia burgdorferi , named MH 1 strains , and Giemsa staining , scanning electron gold and darkfield microscopy morphology and activity of this strain and monoclonal antibodies were identified by experiments the MH 1 strains with H 5332 , H 9724 positive reaction with H 6931 negative reaction , confirmed that the separation of the spirochete Borrelia burgdorferi . By indirect fluorescent seroepidemiological survey results confirmed that the the survey points cattle and voles Borrelia burgdorferi infection , the average infection rate of 10% ( 1 / 10 ) and 20 % ( 1 /5 ) The local population are higher than average rate of infection ( 09 %) [ 7 ] < / sup> . The above study confirmed infected with the Xilinguole people and livestock and wild animals have Lyme disease spirochetes . The isolated MH 1 strains pathogenic proved of the strains can make mice suffering varying degrees of a small rabbit skin hyperemia , arthritis , lesions of parenchymal organs .

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Basic Veterinary Science > Animal Microbiology ( Veterinary Microbiology, ) > Pathogenic bacteria
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