Dissertation > Excellent graduate degree dissertation topics show
Identification of Actinomycete SPRI-38490 and the Study of Its Active Metabolites
Author: SuChao
Tutor: TaoLiMing
School: East China University of Science and Technology
Course: Pesticides
Keywords: Identification ferment isolation and purification structure identification HPLC herbicidin A
CLC: S482.49
Type: Master's thesis
Year: 2010
Downloads: 59
Quote: 0
Read: Download Dissertation
Abstract
|
Actinomycete SPRI-38490 which could produce herbicidal active metabolites was found by bioscreening. The strain was identified and its herbicidal metabolites were studied.The strain was identified by 16S rDNA gene sequence analysis and the study of morphological, physiological and biochemical characteristics. The results showed that the strain SPRI-38490 belonged to Streptomyces nodosus. With the help of silica gel chromatography, thin layer chromatography, and high performance liquid chromatography and other ways, a purified compound with herbicidal activities was isolated from the fermentation broth, named SPRI-38490-A. The spectra data of UV, MS,1H-NMR,13C-NMR and others were used to identify this compound. The molecular formula of the compound was confirmed as C23H29N5O11, with a molecular weight of 551. Compared with the known compounds in literatures and compounds database, the structure of SPRI-38490-A was as same as herbicidin A. Its herbicidal activities were proved. The reverse phase high-performance liquid chromatography analysis method was established, which could be used to analyse herbicicin A in the process of the fermentation.Compared with the known strains which could produce herbicidin A, the strain SPRI-38490 was different with any of them. The other strains produced other components of herbicidin while producing herbicidin A, but only herbicidin A was found in the metabolites of the strain SPRI-38490.
|
Related Dissertations
- Temperature Drift Modeling and Compensation of Fiber-Optic Gyroscopes,V241.5
- Simulation and Analysis about Switched Reluctance Generator Power Supply System,TM31
- Research on Vector Control System of Induction Motor Based on DSP,TM346
- Compensation Methods of Different Speech Coding for Speaker Recognition,TN912.34
- Design and Simulation of UHF RFID System Based on the Protocol of EPC C1G2,TP391.44
- The Software Component Modeling Method Based on Feature and Its Application in VMI Management System,TP311.52
- The Study of Domain Dictionary Construction Based on Web,TP391.1
- Research on the Control and Remedy of Nanopositioning Stage Drived by Piezo Material Lead Zirconate Titanate,TP273
- Studies on Isolation, Purification and Chemical Structure for Polysaccharides from Camellia Chrysantha(Hu) Tuyama,S567.19
- Aerial Target Anti-interference Recognition and Tracking System,TN215
- Study on the Pharmacokinetics of Xiaoaiping and Chlorogenic Acid in Rats and the Quality Standard of Xiaoaiping Preparations,R285
- The Study on Rhinoceros Horn and Its Imitation,TS932.2
- Characterization of Corn Lectin and Application to Screen of Growth-promoting Bacteria from Corn Rhizosphere,S513
- Study on Antioxidant Components of Tibetan Medicine Sanguo Tang San,R29
- Isolation Entophytes and Study on the Fermentation of Bacillus Subtilis tiD7 from Rehmannia,TQ461
- Over-voltage Identification of Transmission Line Based on Hilbert-Huang Transformation,TM866
- Study of Synchronous Generator Excitation Control Based on Neural Network Identification,TM31
- Survey in Nanjing and Analysis of the Mitochondrial Genome of Frankliniella Occidentalis (Pergande),S433
- Research on Peer-to-Peer Traffic Identification Algorithm Based on Cluster Analysis,TP393.02
- Animal products quality safety and security regulation of RFID systems,TS201.6
- The Identificationand Rapdanalysis of Wheat Root Lesion Pathogens in Henan Province,S435.121
CLC: > Agricultural Sciences > Plant Protection > Pesticide ( chemical control ) > Various pesticides > Herbicides ( herbicide ) > Other herbicides
© 2012 www.DissertationTopic.Net Mobile
|